1 You obtained the following raw data when setting up a Biuret standard curve:BSA (mg/ml)012345678910Absorbancy540nm0.1580.2100.2600.3050.3600.4100.4550.5100.5300.5500.554After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and anabsorbancy of 0.310 was obtained.Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (upto 3 significant figures).

Answered: Image /qna-images/answer/505fe4ca-67ac-4954-8cb6-9129ce51bfde.jpg

absorbancy of 0.310 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).

absorbancy of 0.310 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter22: Proteins

Section: Chapter Questions

Problem 22.69P

See similar textbooks

Related questions

Q: Ammonia (NH3) gas and oxygen (O₂) gas react to form nitrogen (N₂) gas and water (H₂O) vapor. Suppose…

Q: Q/Write the chemical structure of the polymers 1- poly(2-propyl ethyl acrylate) 2- poly(1-phenyl…

A: A question based on IUPAC nomenclature that is to be accomplished.

Q: Н. Н.

Q: The value of E°cell for a particular electrochemical cell is +1.44 V. This is a/an ________________…

A: The value of E°cell for a particular electrochemical cell is +1.44 V. This is a/an ________________…

Q: Which of the following terms apply to valine at pH 7.0? a. Amphipathic b. Zwitterion c. Diprotic d.…

A: Term applicable for valine

Q: 1. Draw a Lewis structure; fill in any nonbonding electrons. 2. Calculate the formal charge on each…

A: Formula for formal charge calculation., Formal Charge = Number of Valence electron - Number of lone…

Q: That is the correct name for this molecule? Remember to clude the isomerism in the name. nswer: |

Q: The production of high-grade iron from hematite and magnetite using a blast furnace involves several…

A: The production of high-grade iron from hematite and magnetite using a blast furnace involves several…

Q: A. Na2S+CaCl2 CaS + Na → C. Zn(s) + H₂SO4 → ZnSO4+H2 1 B. Cu (g) → Cu²(g) + le D. Cu(g) → Cu¹*(s) +…

A: Ionization energy (EI): When an electron is removed from the outermost shell of an isolated gaseous…

Q: a) Dissociation of sodium chloride b) Addition of hydroxide ion to acetone c) Formation of potassium…

A: The electron pushing arrows, bonds and lone pairs are shown in the following step.

Q: Write the chemical formula of the compounds in letters 1- poly(2-propyl ethyl acrylate) 2-…

Q: Lead (II) bromide, PbBr2, has Ksp= 4.6 x 10–6. (a) Will precipitate of PbBr2 form when 20.0 mL of…

A: (a) The concentration of Pb2+ after mixing = 0.10 M×20.0ml20.0ml +30.0 ml…

Q: London forces are forces of attraction between what molecules. a. polar b. nonpolar c. slightly…

Q: Give the conjugate base of each of the following: 1. HNO3 2. CH3COOH 3. HPO42-

A: Given species: 1. HNO3 2. CH3COOH 3. HPO42- We have to write the conjugate bases of these species.

Q: Provide the complete mechanism using curved arrow formalism for the reaction of isopropylbenzoate…

A: Sodium benzoate can be separated from isopropyl alcohol by acidic hydrolysis. Benzoic acid is…

Q: Which of the following pairs are constitutional isomers? O 3-methylpentane and 2-methylbutane O…

Q: Reaction of Benzoic acid and ethanol under acid catalysis is an example of formation of Aldol…

Q: 3. Indicate which arrow-pushing schemes below show proper use of curved arrows and which do not.…

A: Concepts of the organic reaction mechanism.

Q: For the following reaction, 0.522 moles of iron(II) chloride are mixed with 0.286 moles of silver…

A: Given that, FeCl2 + 2 AgNO3 →FeNO32 + 2AgCl Moles of iron chloride = 0.522 moles Moles of Silver…

Q: A galvanic cell needs to be set up so that the reduction of Sn4+ to Sn2+ takes place at the cathode.…

A: A galvanic cell needs to be set up so that the reduction of Sn4+ to Sn2+ takes place at the cathode.…

Q: Multiple response question, 15 % Penalty for each incorrect responses. DO NOT GUESS, it may reduce…

A: Concept based on proton NMR spectrum.

Q: 2-35 Predict which compound in each pair has the higher boiling point. Explain your prediction. (a)…

A: Please find the attachment.

Q: H- HHH C HHH I_C|I - C ·C· ·CH Identify and select all structures below that represent a…

Q: С о CH₂CCH₂CH₂-C-OH CH₂CH3 CI IUPAC name:

Q: Which of the following organisms is the causative agent for skin infections and toxic shock…

A: Which organism is the causative agent for skin infections and toxic shock syndrome?

Q: When a potential difference of 150.0 V is applied to the plates of a parallel-plate capcitor, the…

Q: how many hydrogen bond acceptors are there in pantoprazole?

A: If we consider any hydrogen bond, there consists a donor and an acceptor part. The donor part is the…

Q: 2. Element "X" has 2 naturally occurring isotopes with the following masses and natural abund X-69…

Q: Consider the alkane, n-propane. What is the hybridization of the 3 carbon atoms? O the carbon atoms…

Q: What is the molar concentration, millimolar (mM) concentration, and micromolar (µM) concentration…

A: moles of glycine = 3 micromoles (µmoles) = 3 x 10-6 moles volume = 6 L

Q: 21.9 Identify reagents that can be used to accomplish each of the following transformations: a) OH…

A: Organic reactions are those in which organic reactant react to form organic products.

Q: Which of the following sequences ranks the structures below in order of increasing acidity? OH Дон 1…

A: The stability of the heterocyclic compounds such a pyrrole and pyridine can be directly correlated…

Q: solute

Q: Starting with the alkyne A, provide a logical stepwise strategy for the synthesis of allylic alcohol…

A: Organic transformation.

Q: What are the functional groups in Organic chemistry? Write their structures and give at least three…

A: functional groups in Organic chemistry

Q: calculate the concentration of lead ion in saturated solution of lead chloride dissolve in 0.10 M…

A: Given : Ksp PbCl2 = 1.8×10-4 Concentration of lead chloride dissolve in 0.10 M NaNO3 = ?

Q: Ex. What is the molar concentration of AgNO3 solution standardized against 712 mg primary standard…

Q: 21.11 Identify reagents that can be used to accomplish each of the following transformations (you…

Q: g CH3 T CH3-CH2-C-CH2 - CH3 h I CH₂ h I CH3 CH3 CH3 I T CH3-CH2-C- C- CH2 - CH2 - CH3 I I CH3 CH3…

A: IUPAC name of the above given molecules are shown below;

Q: The brick-red color observed when Benedict's test is applied to D-mannose is due to the reduced form…

A: -> Benedict's tast is a chemical test that can be used to check for the presence of reducing…

Q: (structure below) is proposed as a model compound for an application of the particle-in-a-ring…

A: Please find the attachment.

Q: & = & D HO OH

Q: НО. E F OH

Q: Given the following reaction: 4 HNO3(aq) + Cu(s) → Cu(NO3)2(aq) + 2 NO₂(g) + 2 H₂O(1) Calculate the…

Q: X₂ X Ω. Perform the following operations giving the proper number of significant figures in the…

Q: Calculate the IHD of C7H6XNO and identify the important peaks in the following MS spectral data and…

Q: 1 i) A first-order reaction has a half-life of 22 seconds. a) Calculate the rate constant for this…

A: Given, a first order reaction with its half-life and initial concentration of reactant.we are asked…

Q: Draw the most stable conformation of the disubstituted cyclohexane below

A: Conformational isomers: The conformational isomers are formed by the rotation of a carbon-carbon…

Q: НО

A: The hyberdisation of each carbon in the molecule is written in the bracket.

Q: (structure below) is proposed as a model compound for an application of the particle-in-a-ring…

Question

You obtained the following raw data when setting up a Biuret standard curve:
BSA (mg/ml)
0123456789
10
Absorbancy
540nm
0.158
0.210
0.260
0.305
0.360
0.410
0.455
0.510
0.530
0.550
0.554
After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an
absorbancy of 0.310 was obtained.
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up
to 3 significant figures).

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step 3

VIEW

Step by step

Solved in 3 steps with 2 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

A student added a total of 75 mL sodium hydroxide to titrate an amino acid solution with 0.5M NaOH. They also calculated that they added a total of 14 mL of sodium hydroxide to arrive at pH 2.6 and a total of 32 mL of sodium hydroxide to arrvive at pH 4.8.What was the initial concentration of the amino acid solution in milimolar (mM)? The calculations must be presented such that those can be recapitulated by the marker. Provide the answer with no decimal places. Show all details of your working out. State the answer in a full sentence.
q41 IF YOU DO NOT KNOW THE ANSWER PLEASE DO NOT REJECT IT please calculate the unknown concentration of the protein D wih an absorbance value of A412 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762
q42 please calculate the unknown concentration of the protein D wih an absorbance value of A554 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762
The amino acid glycine (H2N—CH2—COOH) can participatein the following equilibria in water:H2N—CH2—COOH + H2O ⇌H2N—CH2—COO- + H3O+ Ka = 4.3 x 10-3 H2N—CH2—COOH + H2O ⇌+H3N—CH2—COOH + OH- Kb= 6.0 x 10-5(a) Use the values of Ka and Kb to estimate the equilibriumconstant for the intramolecular proton transfer to forma zwitterion:H2N—CH2—COOH ⇌ +H3N—CH2—COO-(b) What is the pH of a 0.050 M aqueous solution of glycine?(c) What would be the predominant form of glycine in asolution with pH 13? With pH 1?
Ksp for BaCrO4 is 1.17x10^-10
The denaturing of proteins occurs when the stabilizing forces are altered. Below is the set of processes in which proteins are denatured. Classify the following processes according to the denaturing agent involved. Please help me understand the diff and correct any mistakes.
The activity coefficient of aqueous glucose (C6H12O6) is ______. cannot be estimated from the given information slightly more than 1 slightly less than 1 much less than 1 0 exactly 1
I conducted an experiment using a glucometer with a lancet. Solutions were prepared to measure the glucose level. It was successful, however when fruit juice was measured it gave the most incarnate reading of them all. What can be done to improve the sensor performance for the analysis of the sugar content in juice?
In replicate analyses, the carbohydrate content of a glycoprotein (a protein with sugars attached to it) is found to be 12.6, 11.9, 13.0, 12.7, and 12.5 g of carbohydrate per 100 g of protein. Find the 50% and 90% confidence intervals for the carbohydrate content.
In the preparation of the standard curve for protein analysis, 50 mn BSA (bovine serum albumin) dissolved in H2O to a final volume of 5 mL was used as stock solution. What is the weight of BSA in 0.1 mL of stock solution? In 0.2 mL?
5 optimise, produce and market a commercial assay kit for the enzyme-linked determination of glucose in a range of samples
Consider the following data that was collected for a protein that contain 0.76 % tryptophan amino acids by weight (Molecular weight of tryptophan amino acids is 204 g/mol). Absorbance Concentration (mg/L) 0.0250 0.100 0.150 0.226 0.259 0.400 0.600 0.964 1.14 1.78 1.45 2.35 1.78 2.87 How many mg of the protein present in 1.00 L solution that shows an absorbance of 1.20?