Acid phosphatases are an important group of enzymes that can be detected in human blood serum.Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyze biological phosphate esters as follows: R-O-P-O3-2 + H 2O R-OH + HO-P-O3-2. Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver, and prostate gland. The enzyme from the prostate gland is clinically important because an increased activity in the blood is frequently an indication of cancer of the prostate gland. Tartrate ion can strongly inhibit the phosphatase from the prostate gland, but not acid phosphatases from other tissues. How canyou use the information above to develop a specific procedure for measuring the activity of the acid phosphatase of the prostate gland in human blood serum?
1. Acid phosphatases are an important group of enzymes that can be detected in human blood serum.Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyze biological phosphate esters as follows:
R-O-P-O3-2 + H 2O R-OH + HO-P-O3-2.
Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver, and prostate gland. The enzyme from the prostate gland is clinically important because an increased activity in the blood is frequently an indication of cancer of the prostate gland. Tartrate ion can strongly inhibit the phosphatase from the prostate gland, but not acid phosphatases from other tissues. How canyou use the information above to develop a specific procedure for measuring the activity of the acid phosphatase of the prostate gland in human blood serum?
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