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- A person with cancer walks into the classroom. As a biology class, we ask if we can examine some of the cancerous cells and find that many of these cancerous cells have an extra chromosome 10 (trisomy) leading to more cyclin for forming the MPF being made, leading to uncontrollable cell division (i.e. tumor and progression to cancer). What would cause extra chromosomes in cells (be it somatic or gametic cells)? Select one: a. None of these choices are linked to how sister chromatids separate and would lead to extra chromosomes in daughter cells of cell division. b. APC not functioning correctly to add ubiquitin to the cohesins, thus sister chromatids do not split correctly during cell division c. MPF itself would cause more chromosome replication d. APC is overly functioning adding cohesins to the sister chromatidsLet’s suppose an X-linked gene in mice exists as two alleles, whichwe will call B and b. X-chromosome inactivation, a process inwhich one X chromosome is turned off, occurs in the somatic cellsof female mammals . Allele B encodes an mRNA that is 900 nucleotides long, whereas allele b contains a small deletion that shortens the mRNA to a length of 825 nucleotides.Draw the expected Northern blot that will be obtained usingmRNA isolated from somatic tissue of the followingmice:Lane 1. mRNA from an XbY male mouseLane 2. mRNA from an XbXb female mouseLane 3. mRNA from an XBXb female mouse.Note: The sample taken from the female mouse is not from aclone of cells. It is from a tissue sample, like the one shown.1. a)Proteins that stimulate/promote progression through the cell cycle are encoded by (oncogenes or tumor suppressor genes). Boldface one. b)Proteins that inhibit progression through the cell cycle are encoded by (oncogenes or tumor suppressor genes). Boldface one. c)What is the difference between a proto-oncogene and an oncogene? d)To cause cancer, proto-oncogenes require (1 or 2)allele(s) to be mutated and therefore are considered (dominant or recessive). The mutation results in a (loss or gain) of function. For each underlined pair, boldface one. e)To cause cancer, tumor suppressor genes require (1 or 2)allele(s) to be mutated and therefore are considered (dominant or recessive). The mutation results in a (loss or gain) of function. For each underlined pair, boldface one.
- An individual has a deletion of part of the short arm of chromosome 5. This individual also has a normal copy of chromosome 5. Would you expect this individual to have Cri-du-chat syndrome? Why or why not? Question 7 options: A) Yes, because they will inactivate their normal chromosome 5 B) Yes, because they only contain one copy of some of their genes C) No, because this is a terminal deficiency D) No, because they still have at least one copy of all the genes on chromosome 5Equalizing the Expression of X Chromosome Genes in Males and Females Males have only one X chromosome and therefore only one copy of all genes on the X chromosome. Each gene is directly expressed, thus providing the basis of hemizygosity in males. Females have two X chromosomes, but one is always inactivated. Therefore, females, like males, have only one functional copy of all the genes on the X chromosome. Again, each gene must be directly expressed. Why, then, are females not considered hemizygous, and why are they not afflicted with sex-linked recessive diseases as often as males are?The dominant condition elliptocytosis causes red blood cells to become misshapen into oval-shaped cells. One of the genes responsible for the abnormal shape encodes the band 4.1 protein that together with ankyrin and other scaffold proteins creates and maintains the spherical concave shape of a normal red blood cell. The gene for band 4.1 protein, EPB41, is found on the p arm of chromosome 1. This is very close to the gene encoding the red blood cell Rhesus (Rh) blood type, either phenotype + (dominant) or - (recessive), with a recombination frequency of 2%. This means that 98% of the time alleles for these two genes are linked and are transmitted together. Diane and Jack are siblings, and both have elliptocytosis and Rh+ blood type. Due to the elliptocytosis, both had emergency splenectomies after having severe anemia. Their younger brother, Devonté, has not yet shown signs of elliptocytosis, but has Rh- blood. André, their dad, also has elliptocytosis and Rh+ blood; while their…
- 1. During the Human Genome Project, scientists learned that most of the human genome A. doesn't code for proteins. B. is located on sex chromosomes. C. isn't inherited. D. codes for proteins. 2 Tortoiseshell cats have different alleles for fur colors on their X chromosomes, with different X chromoson bein activate different cells, a phenomenon called A X-inactivation. B. X-expression. C. X-activation. D. Y-inactivation. 3 Consider that people with blue eyes must have two alleles for blue eyes since blue eye color is a recessive trait. A blue-eyed person and a brown-eyed person have an offspring with blue eyes. The brown-eyed person must A. have two alleles for blue eyes. B. have two alleles for brown eyes. C. not have the dominant brown eye allele. D, have one allele for blue eyes and one allele for brown eyes.You are working in a cell biology lab that investigates non-small cell lung cancer cells, which of these cellular features will be suggestive of senescence in the cells observed? Choose all that apply: Group of answer choices Large flattened morphology Reduced incorporation of 5-bromodeoxyuridine (in DNA replication) Increased p53 expression Decreased expression of p15INK4BDiseases which are caused by recessive variants in loci located on the X chromosome affect females and males differently. How? Lifestyle choices and environmental exposures can also shape up a disease, how? (Discuss from the aspects of molecular biology) c. In a designed Microarray experiment, in which the probes for the control cell’s transcripts are labelled with green dye and probes for cancer cell’s transcripts are labelled with red dye. What color intensities will you expect in the spots of oncogenes and tumor suppressor genes?
- Following X-chromosome inactivation, most of the genes on theinactivated X chromosome are silenced. Explain how. Name onegene that is not silenced.Explain about how X chromosome inactivation takes place in mammals at the chromosomal and molecular level. B. What genes are involved in inactivating a human X chromosome? . C. What role does X chromosome-specific inactivating transcript --XIST-- non coding RNA play in the inactivation of the human X chromosome.Figure 19.25 shows that the Sxl protein binds to the mRNA of the msl-2 gene, inhibiting translation of the mRNA’s proper reading frame. The MSL-2 protein is a transcription factor that binds to the X chromosome in XY males to double the level of X-linked gene transcription, thus equalizing X-linked gene expression in XY males and XX females. a. In which sex, XY males or XX females, would the Sxl protein bind to the msl-2 mRNA? b. As discussed in Problem 35, some Sxl alleles are lethal to females and others are lethal to males. Is the function of Sxl in regulating the synthesis of Msl-2 protein sufficient to explain the sex-specific lethality caused by both kinds of alleles? c. Predict the effect of loss-of-function mutations in msl-2 on male and female fertility and viability.