Agarose gel Can someone help me to understand how to estimate the size of my plasmid cut (pla cut) and insert (PCR cut) that shows in the picture?    thank you    (its from an cloning experiment)

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Agarose gel

Can someone help me to understand how to estimate the size of my plasmid cut (pla cut) and insert (PCR cut) that shows in the picture? 

 

thank you 

 

(its from an cloning experiment)

bp ng/0.5 µg %
20000 20 4
10000 20 4
7000 20 4
5000 75 15
4000 20 4
3000 20 4
2000 20 4
1500 80 16
1000 25 5
700 25 5
500 75 15
400
25 5
300
200
75
25 5
25
25
0.5 µg/lane, 8 cm length gel,
1X TAE, 7 V/cm, 45 min
MW Pla Pla PCR PCR
marker cut uncut cut uncut
>
}
Transcribed Image Text:bp ng/0.5 µg % 20000 20 4 10000 20 4 7000 20 4 5000 75 15 4000 20 4 3000 20 4 2000 20 4 1500 80 16 1000 25 5 700 25 5 500 75 15 400 25 5 300 200 75 25 5 25 25 0.5 µg/lane, 8 cm length gel, 1X TAE, 7 V/cm, 45 min MW Pla Pla PCR PCR marker cut uncut cut uncut > }
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