An 89 year old man presented to a community hospital emergency room with weakness and inability to walk. Among other tests, two sets of blood cultures were drawn to rule out sepsis, and submitted to the Microbiology laboratory where they were put into an automated blood culture instrument for incubation. Within twenty four hours both aerobic bottles and one anaerobic bottle flagged as positive. Aerobic bottles were subcultured to Sheep Blood, Chocolate and MacConkey agar plates incubated at 35-37C in 5-10% CO2, as well as an anaerobic Brucella agar plate incubated anaerobically. Anaerobic subculture included a BBE/LKV plate incubated anaerobically. Direct and Day 1 gram stains showed small, non-spore forming gram positive rods. Gram stain of culture Plates were examined on Day 1. A small, whitish, moist colony with a narrow zone of beta hemolysis around and under the colonies was noted on Blood agar plates. Left: blood agar plate; Right: same plate with transmitted light Growth was noted on Chocolate and anaerobic Brucella agar plate. A Bile Esculin slant was inoculated and turned positive the next day. A wet mount was done at the bench and the organism appeared very motile with a characteristic type of "tumbling" motility. The catalase test was positive. A CAMP test was performed; however, results were questionably negative. An automated identification system identified the organism at the 99% level. What is the organism?
An 89 year old man presented to a community hospital emergency room with weakness and inability to walk. Among other tests, two sets of blood cultures were drawn to rule out sepsis, and submitted to the Microbiology laboratory where they were put into an automated blood culture instrument for incubation. Within twenty four hours both aerobic bottles and one anaerobic bottle flagged as positive. Aerobic bottles were subcultured to Sheep Blood, Chocolate and MacConkey agar plates incubated at 35-37C in 5-10% CO2, as well as an anaerobic Brucella agar plate incubated anaerobically. Anaerobic subculture included a BBE/LKV plate incubated anaerobically. Direct and Day 1 gram stains showed small, non-spore forming gram positive rods. Gram stain of culture Plates were examined on Day 1. A small, whitish, moist colony with a narrow zone of beta hemolysis around and under the colonies was noted on Blood agar plates. Left: blood agar plate; Right: same plate with transmitted light Growth was noted on Chocolate and anaerobic Brucella agar plate. A Bile Esculin slant was inoculated and turned positive the next day. A wet mount was done at the bench and the organism appeared very motile with a characteristic type of "tumbling" motility. The catalase test was positive. A CAMP test was performed; however, results were questionably negative. An automated identification system identified the organism at the 99% level. What is the organism?
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter18: Instrumentation In The Operating Room
Section: Chapter Questions
Problem 3CS
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Question
Please identify the organism.
Transcribed Image Text:An 89 year old man presented to a community hospital emergency room with weakness and inability to walk.
Among other tests, two sets of blood cultures were drawn to rule out sepsis, and submitted to the Microbiology laboratory where
they were put into an automated blood culture instrument for incubation.
Within twenty four hours both aerobic bottles and one anaerobic bottle flagged as positive. Aerobic bottles were subcultured to
Sheep Blood, Chocolate and MacConkey agar plates incubated at 35-37C in 5-10% CO2, as well as an anaerobic Brucella agar
plate incubated anaerobically. Anaerobic subculture included a BBE/LKV plate incubated anaerobically.
Direct and Day 1 gram stains showed small, non-spore forming gram positive rods.
Gram stain of culture
Plates were examined on Day 1. A small, whitish, moist colony with a narrow zone of beta hemolysis around and under the
colonies was noted on Blood agar plates.
Left: blood agar plate; Right: same plate with transmitted light
Growth was noted on Chocolate and anaerobic Brucella agar plate. A Bile Esculin slant was inoculated and turned positive the
next day.
A wet mount was done at the bench and the organism appeared very motile with a characteristic type of "tumbling" motility. The
catalase test was positive. A CAMP test was performed; however, results were questionably negative.
An automated identification system identified the organism at the 99% level.
What is the organism?
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