An enzyme catalyzes the reaction A ⇌ B. The enzyme is present at a concentration of 2 nM, and the Vmax is 1.2 μM s −1. The Km for substrate A is 10 μM. Calculate the initial velocity of the reaction, V0 , when the substrateconcentration is (a) 2 μM, (b) 10 μM, (c) 30 μM.

Given information: Vmax is 1.2 μM s −1 Km = 10 μM

Answered: An enzyme catalyzes the reaction A ⇌ B.…

Chemistry: Principles and Practice

3rd Edition

ISBN:9780534420123

Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer

Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer

Chapter13: Chemical Kinetics

Section: Chapter Questions

Problem 13.85QE

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An enzyme catalyzes the reaction A ⇌ B. The enzyme is present at a concentration of 2 nM, and the Vmax is 1.2 μM s ⁻¹. The K_m for substrate A is 10 μM. Calculate the initial velocity of the reaction, V₀ , when the substrate
concentration is (a) 2 μM, (b) 10 μM, (c) 30 μM.

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