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- A student performs a separation by TLC on a silica gel platee that results in three spots. A red spot is measured 23 mm away from the original sample spot, a blue spot is 42 mm away, and a yellow spot is 49 mm away. the distance from the original spot to where the solvent stopped is 55 mm. a) calculate the Rf value for each componenet in the mixture (show work and report th correct number of significant figures) b) the silica gel plate is polar. rank the three componenets in the mixture from lowest to highest polarity. Explain your answer.What is the balanced equation for the combustion of C8H7NO2SBrCl in aC,H,N,S elemental analyzer?Define the following terms used in HPLC: (d) sampling loops (e) bonded-phase packing (f) gel filtration
- In a normal phase chromatography set up, the component that yields the highest Rf value is likely to be _____. Group of answer choices Both polarity Cannot be determined Polar Non-polar2 d) In a typical HPLC column, 150 atm of pressure is required to push the mobile phase through the column. Why is high pressure needed in HPLC? e) Why are HPLC particles porous?It refers to the general term of adsorbent media used to visualize chromogenic compounds.A. ChromatogramB. Solvent Front C. Mobile PhaseD. Stationary Phase