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Principles Of Pharmacology Med Assist

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter12: Parenteral Equipment And Supplies

Section: Chapter Questions

Problem 24RQ

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EFFECT OF ENZYΜΕ CONCENTRATION OΝ ENZΥME ACTIVITY
Question Guide:
1. Approximately what is the concentration of the enzyme in each tube? Show computation
Tube No.
Computation
1
2
3
4
5
6.

Procedure:
1. Prepare 6 test tubes and place 1 ml saliva in each.
2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to
tube 5 and 10 ml to tube 6. Mix thoroughly.
3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and
add 1 ml of 1% starch paste. Mix well and heat in a water bath with
temperature maintained at 40°C for 30 minutes.
4. Divide each of the contents of each tube into 2 and test with:
a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI
and note the color produced. Compare the intensity of color in each of the
6 tubes.
b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of
each tube and heat in a boiling water bath for 3 minutes. Note color of
precipitate.
5. Rank each tube according to decreasing reaction rate one (1) being the fastest
to hydrolyze and 6 being the slowest.

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