DNS reaction is an alternative assay for enzyme activity .What is involved or basis for this reaction?
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DNS reaction is an alternative assay for enzyme activity .What is involved or basis for this reaction?
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- Briefly comment on the differences of using a fixed-time assay versus a kinetic assay to measure enzyme activity. Is it reasonable to assume that the reaction velocity obtained by measuring the amount of product after 30 minutes in a fixed-time assay is directly proportional to absorbance? How could you determine whether this was the case? Word limit 180 words including citation and referenceWhat is the difference between the lock-and-key model of enzyme action and the induced-fit model?What is the interpretation and reaction catalyzed of this GPT enzyme test?.
- What are the four key types of irreversible inhibitors that can be used to study enzyme function?For the following problem, indicate whether enzyme is repressed (-) or produced (+). Please show all possible outcomes.For most physiological/biochemical experiments, it is usually most desirable for cells to be in the • A. exponential phase • B. death phase C. lag phase • D. stationary phase
- Does the presence of an uncompetitive inhibitor increase / decrease the apparent affinity of the enzyme•substrate complex?Does the apparent KM increase / decrease in the presence of an uncompetitive inhibitor?What is the difference between lock-and-key and induced-fit models for binding of a substrate to an enzyme?Why must enzyme activity be monitored under standard conditions?