Assay # 1 2 3 4 PNPP, conc. μΜ 150 75 37.5 18.75 Volume of 4 mM PNPP, mL 0.15 0.075 0.0375 0.01875 Volume of 0.2 M Tris pH 8, mL 0.4 0.4 0.4 0.4 Volume of H₂O, mL 3.35 3.43 3.46 3.48 Volume of 1/4 enzyme solution, mL 0.100 0.100 0.100 0.100 Total volume, mL 4 4 4 4
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What is the extinction coefficient for the following absorbances?
1. 3.000
2. 2.022
3.1.2025
4. 0.824
Would the units be in µmoles/mL)^-1 cm^-1
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- You are given three unknown samples stored in screw-cappedcontainers and was told to analyse them. You noted that Container A iscloudy and have some particles that are staying near the bottom of thecontainer. Container B is translucent while Container C is transparent. If thecontents of Container A can be separated by simple decantation,what isthe particle size that BEST describes its composition?i. <1 nmii. 1 nmiii. 1 nm-100 nmiv. >1000 nmMass of aspirin used is .090 g the absorbance for the final solution was .304 nm Slope is 1708 and y-intercept is -0.03397 what is the molarity of the final unknown solution and the molarity of the original solution made from aspirin in the 250.0 ml flaskA. B. and C. already solved (https://www.bartleby.com/questions-and-answers/chemistry-question/a89691d6-2162-4677-b0cc-dc84fcceda08). Please answer E, F and GCircle in pencil in the image on th eirght cide of the diagram is a "2"( Pb(NO3)2 ).
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- Please create a caption for this table. Solution NaCl Conc. (%) Osmolality (mOsm) % transmittance Absorbance % hemolysis % crenation C distilled 0 0 0.001029 4.987584625 100 0.03354 1 0.177179111 54.61 0.001551 4.809388202 96.42720001 0.05837 2 0.297126222 91.58 0.01012 3.994819487 80.09527231 0.08444 3 0.442542222 136.4 3.849 1.414652089 28.3634704 0.134 4 0.590164444 181.9 64.8 0.188424994 3.777880643 0.2125 5 0.74752 230.4 95.64 0.019360433 0.388172513 0.3368 6 0.89644 276.3 99.56 0.001915112 0.038397585 0.5336 7 1.095648889 337.7 99.98 8.68676E-05 0.001741676 0.9834 8 1.336711111 412 100 0 0 2.1 9 1.755568889 541.1 100 0 0 7.9 10 2.674395556 824.3 100 0 0 57.83 11 4.490211111 1384 100 0 0 99.72A chemist obtained the following data for the percent compound Z in triplicates (n=3) of an insecticide preparation: 7.47, 6.98, and 7.27. Calculate the 90% confidence limit for the mean of the data assuming that only information about the precision of the method is the precision for the three data points.#9 part E
- What happen to the separation raid (RS) I change the mobile phase from methanol/water 70/30 till methanol/water 80/20. How does polarity affect?Discuss the language demands included in the lesson on mixing substances in a 5th grade classroom by Identify the language function(s) based on the below standard and learning objective. Standard Conduct an investigation to determine whether the mixing of two or more substances results in new substances. Learning Objective Given a variety of substances and safety equipment in a classroom setting, students will be able to conduct an experiment to mix two or more substances and observe the results. They will be able to correctly identify whether a new substance has been formed as a result of the mixing, based on observable changes such as color change, formation of a precipitate, or change in temperature, with at least 80% accuracy.A solution of potassium permanganate of unknown concentration was analyzed against a standard of various concentrations. Refer to the following data set to answer the succeeding questions (MM KMnO4 = 158.034 g/mol). Table 1. Data for Standard Calibration Curve KMnO4 Standard (grams/Liter) Absorbance at 525 nm 0.008 0.10437 0.158 0.20197 0.316 0.45288 0.474 0.68232 0.632 0.89361 Table 2. Data for KMnO4 Solution of Unknown Conc. Sample of Unknown Conc. Absorbance at 525 nm Undiluted KMnO4 0.95423 Diluted KMnO4 (50% dilution) 0.46514 Which of the following linear equation for the standard calibration curve is generated fromTable 1? y= 0.03231x – 0.74923 y= 0.04848x + 1.3179 y= 1.3179x + 0.04845 y= 0.74923x – 0.03231