Assume that a cycle of the polymerase chain reaction takes 20 minutes. How many copies of a single DNA fragment will there be after the reaction has been run for six hours?
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Assume that a cycle of the polymerase chain reaction takes 20 minutes. How many copies of a single DNA fragment will there be after the reaction has been run for six hours?
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- which step of the polymerase chain reaction takes place at 98 degrees?Referring to Figure 7-20, answer the following questions:a. What is the DNA polymerase I enzyme doing?b. What other proteins are required for the DNApolymerase III on the left to continue synthesizingDNA?c. What other proteins are required for the DNApolymerase III on the right to continue synthesizingDNA?How many different temperatures are used in each cycle of the polymerase chain reaction?
- Briefly describe the polymerase chain reaction. What is the difference between a step and a cycle?What are the essential requirements for the polymerase chain reactionSuppose DNA polymerase synthesizes DNA at a rate of 1130 bases per minute in a new strain of E. coli. It takes the new strain 40 minutes to replicate their chromosome. How many nucleotides are in the chromosome? Calculate the length of the chromosome given the diagram.
- The synthetic unit of the polymerase chain reaction is the replica. True or false?Does the addition of a histidine tag affect DNA polymerase activity and or processivity? Give a detailed explanation.Considering prokaryotes, what is the enzyme that helps hold DNA polymerase III in place when nucleotides are being added?
- What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that is to be copied?True or false: After chromosomal replication, Type I topoisomerase is used to coil up the double-stranded DNA?Suppose that 28% of the nucleotides in a DNA moleculeare deoxythymidine 5′- monophosphate, and that duringDNA replication the percentage amounts of availablenucleotide bases are 22% A, 22% C, 28% G, and 28% T.Which base would be depleted first in the replicationprocess?
