What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that is to be copied?

Q: What is the name of the DNA duplication process? What is the main enzyme that participates in it?

A: Every living cell divides to form multiple cells. During this division process, there is another…

Q: What causes different linear DNA molecules to migrate at different speeds during electrophoresis?

A: In molecular biology many techniques are used for different purpose like PCR is used to generate…

Q: What is the role of Mg2+ in genomic DNA extraction?

A: DNA extraction refers to the method of isolating and purifying DNA from a cell. It involves…

Q: What is the significance of temperature control in the polymerase chain reaction?

A: A polymerase the chain reaction (PCR) is a technique for making millions of copies of a single DNA…

Q: What separates the base pair at the start of DNAreplication?

A: DNA replication is the process in which DNA makes a copy of itself by copying a double-stranded DNA…

Q: What happens if DNA polymerase fails to correct an error?

A: DNA is a nucleic acid that serves to store genetic information.

Q: The synthetic unit of the polymerase chain reaction is the replica. True or false?

A: Introduction  PCR is the revolutionized technique invented by Karry Mullis in 1983, for which he got…

Q: How many different temperatures are used in each cycle of the polymerase chain reaction?

A: Polymerase chain reaction (PCR): It is a molecular technique used to make copies of desired gene.…

Q: The polymerase chain reaction can only be used to amplify genes that have been cloned and sequenced.…

A: The polymerase chain reaction was originally developed by the American biochemist Kary Mullis. The…

Q: How did Kornberg assess the fidelity of DNA polymerase I in copying a DNA template?

A: Deoxyribonucleic acid (DNA) is a particle made out of two polynucleotide chains that loop around one…

Q: Describe an experimental approach to determining the processivity of a DNA polymerase (that is, the…

A: The processivity is defined as the degree of the addition of an average number of nucleotides prior…

Q: eant by non-classical DNA-dependent DNA polymerases? What roles do they play in our cells?

A: DNA polymerases are enzymes that assemble nucleotides, DNA's building components, to form DNA…

Q: explain why DNA polymerase 3 and not dna polymerase 1 is responsible for replicating the bacterial…

A: Replication is a process where the genome is duplicated into it's exact copy. In bacteria,…

Q: What does it mean to say that extensions by DNA polymerase III proceeds 5' to 3'?

A: DNA replication is a process through which a new copy of DNA is synthesized. DNA polymerase III is…

Q: How do we know if a genomic DNA sequence contains aprotein-coding gene?

A: Only about 1% of DNA contains protein-coding genes; the remaining 99 percent is noncoding. Noncoding…

Q: Why would telomerase be considered a reverse transcriptase?

A: Telomerase: It is also known as a terminal transferase. A telomere present on the end position of…

Q: What is meant by semi conservative replication? How did Meselson and Stahl prove it  experimentally?

A: DNA is the information hub of the cell that contains instructions for the synthesis of proteins. The…

Q: What are the essential requirements for the polymerase chain reaction

A: Polymerase chain reaction (PCR) is the laboratory technique used to amplify specific piece of sample…

Q: What is Taq polymerase and why is it important?

A: DNA Polymerase is a very important enzyme in the process of DNA Replication. It is a process in…

Q: What is the architecture of the DNA polymerases and the replisome?

A: Gene is the unit of heredity. Living organisms contain genetic materials mainly made up of nucleic…

Q: what are some Limitations/sources of error with suitable rationalization that may occur during the…

A: PCR is in vitro method for amplifying defined target DNA sequences present within the source of DNA.…

Q: Do DNA-polymerase enzymes also function as exonucleases?

A: DNA polymerase enzyme is primarily responsible for the synthesis of DNA by the attachment of various…

Q: What is the difference between terminal deoxynucleotidyl transferase from other polymerases?

A: Terminal deoxynucleotidyl transferase(TdT) is a DNA polymerase that is available in youthful pre-B…

Q: What are replication licensing factors? How did they get their name?

A: Replication is a process by which the DNA is duplicated to form two identical copies of DNA using…

Q: What is the enzyme responsible for methylating DNA? How does a drug like doxorubicin affect DNA…

A: DNA is the nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid in which…

Q: What is the difference between primase and DNA polymerase alpha?

A: DNA is a polymer made up of two polynucleotide chains that coil around each other to form a double…

Q: A mutation in which of these proteins will lead to increased mutations in all daughter cells? A.…

A: The mutation is a heritable change that arises due to the alteration of the genomic sequence of an…

Q: How do we know that DNA synthesis is discontinuous on one of the two template strands?

A: DNA replication refers to the process of making two identical copies of a DNA molecule. This…

Q: What will happen if DNA copying mechanisms were to be less accurate?

A: BASIC INFORMATION DNA Replication It is known as copying of the DNA. It takes place in the…

Q: Describe the key steps in the semiconservativereplication of DNA.

A: DNA replication is a process by which a cell duplicates its DNA before its division. Replication of…

Q: In the Meselson–Stahl experiment, which of the three modes of replication could be ruled out after…

A: The central dogma of molecular biology represents the flow of genetic information from DNA to RNA to…

Q: What information do learn from the results of the DNA extraction?

A: Hi! Since you posted more than one question, we will be answering one for you. If you want the other…

Q: What is the role of the metal ions in the polymerase active site?

A: Metal ions participate by direct and indirect pathways in enzyme catalysis and can also stabilize…

Q: What is the transformation factor?

A: The process in which the genetic material formed of the naked DNA (deoxyribonucleic acid), which is…

Q: In genetic transformation, what is meant by the wordcompetence?

A: The process of taking up naked or foreign deoxyribonucleic acid (DNA) from the environment is called…

Q: How does proofreading differ from later DNA repair mechanisms in terms of timing and the proteins…

A: Proofreading done by DNA polymerase.DNA polymerase detect error at the time of replication and also…

Q: What are the requirements for in vitro synthesis of DNA under thedirection of DNA polymerase I?

A: The formation of deoxyribonucleic acid molecules, either naturally or artificially, is known as DNA…

Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.