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b) What glassware and lab equipment would you need?

b) What glassware and lab equipment would you need?

Organic Chemistry
Organic Chemistry
8th Edition
ISBN:9781305580350
Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions
Section: Chapter Questions
Problem 20.25P
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b) What glassware and lab equipment would you need?
Transcribed Image Text:b) What glassware and lab equipment would you need?
1. This question is adapted from Harris 19-D. Carnosine is a dipeptide whose antioxidant properties protect cells from free radicals. Carnosine was determined by derivatization with naphthalene-2,3-dicarboxyaldehyde and cyanide followed by fluorescence detection using excitation at 445 nm and emission at 490. Quantification was by standard addition. To four aliquots of 20-ul cell lysate were added volumes of 100µM carnosine standard to generate final concentrations of 0, 1.0, 2.5, and 5.0 µM added carnosine. Solutions were diluted to 70µl before addition of 15µl of 5 mM naphthalene-2,3-dicarboxyaldehyde and 15µl of 10 mM NaCN. H H2N. OH Carnosine HN. Naphthalene-2,3- dicarboxyaldehyde Cyanide OH Fluorescent product HN. Fluorescence intensity Concentration (uM) of added carnosine in final 100 µL 0.0 0.465 1.0 0.698 2.5 1.029 5.0 1.651
Transcribed Image Text:1. This question is adapted from Harris 19-D. Carnosine is a dipeptide whose antioxidant properties protect cells from free radicals. Carnosine was determined by derivatization with naphthalene-2,3-dicarboxyaldehyde and cyanide followed by fluorescence detection using excitation at 445 nm and emission at 490. Quantification was by standard addition. To four aliquots of 20-ul cell lysate were added volumes of 100µM carnosine standard to generate final concentrations of 0, 1.0, 2.5, and 5.0 µM added carnosine. Solutions were diluted to 70µl before addition of 15µl of 5 mM naphthalene-2,3-dicarboxyaldehyde and 15µl of 10 mM NaCN. H H2N. OH Carnosine HN. Naphthalene-2,3- dicarboxyaldehyde Cyanide OH Fluorescent product HN. Fluorescence intensity Concentration (uM) of added carnosine in final 100 µL 0.0 0.465 1.0 0.698 2.5 1.029 5.0 1.651
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