You performed an isocratic reversed-phase HPLC separation of phenol, toluene and catechol using a DAD detector set at 275nm and obtained the chromatogram shown below. What are the polarities of the mobile phase and stationary phase in this separation? What order would you expect these compounds to elute in? Why? What changes could you make to the mobile phase to decrease the elution time of the last compound and improve its peak shape?
You performed an isocratic reversed-phase HPLC separation of phenol, toluene and catechol using a DAD detector set at 275nm and obtained the chromatogram shown below. What are the polarities of the mobile phase and stationary phase in this separation? What order would you expect these compounds to elute in? Why? What changes could you make to the mobile phase to decrease the elution time of the last compound and improve its peak shape?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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You performed an isocratic reversed-phase HPLC separation of phenol, toluene and catechol using a DAD detector set at 275nm and obtained the chromatogram shown below.
- What are the polarities of the mobile phase and stationary phase in this separation?
- What order would you expect these compounds to elute in? Why?
- What changes could you make to the mobile phase to decrease the elution time of the last compound and improve its peak shape?
- If all three species are present in the analyzed solution at the same concentration, what might account for different in peak height for the first two compounds eluting from the column? What change to the instrument set-up could you make to increase the detector response of this analyte?
Transcribed Image Text:Time (min)
Absorbance at 275nm
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