NameDATASHEETDateLab SectionI was present and performed this exercise (initials)3-5Gram StainOBSERVATIONS AND INTERPRETATIONS1 Record your observations in the table below. Use separate lines for different organisms found in the Gram stain of your gumline.Include a drawing of your own epithelial cells.Cellular Morphology and Arrangement(include a written description and adetailed sketch of a few representative cells)GramCellReactionOrganism or SourceDimensionsColorBL B. subtilis~1X3-4 micron• Notice: most cellsappear purple while afew appear pink (arrow)Review fig 3.46 on page171 to explain thisphenomenon.

Answered: B. subtilis 1X3-4 micron • Notice: most…

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter49: Animal Reproduction

Section: Chapter Questions

Problem 14TYK

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Question

Name
DATA
SHEET
Date
Lab Section
I was present and performed this exercise (initials)
3-5
Gram Stain
OBSERVATIONS AND INTERPRETATIONS
1 Record your observations in the table below. Use separate lines for different organisms found in the Gram stain of your gumline.
Include a drawing of your own epithelial cells.
Cellular Morphology and Arrangement
(include a written description and a
detailed sketch of a few representative cells)
Gram
Cell
Reaction
Organism or Source
Dimensions
Color
BL

B. subtilis
~1X3-4 micron
• Notice: most cells
appear purple while a
few appear pink (arrow)
Review fig 3.46 on page
171 to explain this
phenomenon.

Expert Solution

Step 1

Gram staining is a common technique used to distinguish between two large groups of bacteria based on their different cell wall constituents. Gram staining distinguishes between Gram-positive and Gram-negative classes by painting these cells red or purple. Gram-positive bacteria stain violet due to the presence of a dense coating of peptidoglycan in their cell walls, which preserves the purple crystal with which these cells are stained. Alternatively, Gram-negative bacteria stain red, which is due to the thinner peptidoglycan wall, which does not maintain the purple crystal during the decoloration process.

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