Bacterial techniques, plasmid isolation, digestion and gel electrophoresis Introduction "Introduce" all the techniques used - handling bacteria (why is this important, what is it used for), plasmid DNA (what is it used for, mention antibiotic resistance) - it is the first step in cloning and DNA manipulation, restriction (what are restriction enzymes, why are they used), gel electrophoresis (what's the principle behind the process and applications). Aims of the experiments Methods (you can skip this section as you didn't actually perform the experiments in the lab, but do watch the videos I sent you, they will be useful for your future study) Results and Discussion
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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