Based on the restriction enzyme specificities given below, which will generate blunt ends? Restriction Enzyme Specificities: ... ECORI .GAATTC... .CTTAAG... .GTTAAC... CAATTG. CTGCAG.. GẠCGTC... Hpal PstI Hind III ... AAGCTT... ITCGAA... Bam HI ...GGATCC... ..CCTAGG... A) EcoRI B) BamHI c) Hindll D Hpal E Pstl

Based on the restriction enzyme specificities given below, which will generate blunt ends? Restriction Enzyme Specificities: ... ECORI .GAATTC... .CTTAAG... .GTTAAC... CAATTG. CTGCAG.. GẠCGTC... Hpal PstI Hind III ... AAGCTT... ITCGAA... Bam HI ...GGATCC... ..CCTAGG... A) EcoRI B) BamHI c) Hindll D Hpal E Pstl

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter16: Reproductive Technology, Genetic Testing, And Gene Therapy

Section: Chapter Questions

Problem 12QP: In selecting target cells to receive a transferred gene in gene therapy, what factors do you think...

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Similar questions

During the first successful gene therapy trial in which Ashanti DeSilva was treated for SCID, did the therapeutic gene delivered to Ashanti replace the defective copy of the ADA gene? Why were white blood cells chosen as the targets for the therapeutic gene?
What does it mean if a restriction enzyme produces ‘sticky’ or ‘blunt’ ends? Does the restriction enzyme HaeIII produce “sticky” ends or “blunt” ends?
How many possible restriction maps are compatible with these data?
Explain the process of how X-gal screening works with pUC19, you may build a model with boxes and arrows. 2. You are utilizing BamHI (GGATCC) restriction site and HindIII (AAGCTT) restriction site. Within pUC19, BamHI is at position 263, while HindIII is at position 233. (Hint: position is like coordinate on a map). If you manage to insert GTF2H5 in pUC19 vector, what are the sizes of fragments if you digest the pUC19-GTF2H5 (this is after insertion) with the following restriction enzyme combination after gel electrophoresis: BamHI and HindIII There is an NdeI site right in the middle of the GTF2H5 that has been inserted in pUC19, what would be the fragment sizes, if you digest with BamHI and NdeI. HindIII and NdeI BamHI, HindIII, and NdeI. 3. Design an experiment to confirm the presence of insert GTF2H5 in pUC19 vector using the following method (besides restriction analysis above), assuming that you know the sequence of GTF2H5: Southern Blot Polymerase Chain Reaction
Although SINES are nonautonomous, they do occasionally move from one genomic site to another. How is this usually achieved? a. Using another TE's insertion sequence b. Using another TE's restriction enzyme c. Using another TE's transposase d. Using another TE's reverse transcriptase
Consider the following restriction enzymes, which cut at the positions indicated by arrows: Which enzymes produce ends that are compatible with those of enzyme E? Select all that apply. Enzyme H Enzyme F Enzyme G
In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?
Refer to the diagram of pUC18 (Fig.) to determine which restriction enzymes you could use to insert a gene that would interfere with production of β-galactosidase by the host cell.
For a molecular cloning experiment using the bacterial strain E. coli K12 is used to insert the gene rspL with pUC19. The first attempt utilized the restriction enzyme BamHI which did not produce any transformations. The second attempt utilized the restriction enzymes BamHI and EcoRI. Why was BamHI used first and what difference did the EcoRI do?
When Avery and his colleagues had obtained what was concluded to be the transforming factor from the IIIS virulent cells, they treated the fraction with proteases, RNase, and DNase, followed in each case by the assay for retention or loss of transforming ability. What were the purpose and results of these experiments? What conclusions were drawn?
What is the function of a restriction enzyme?
Which of the following is a sequence at which a restriction enzyme would cut? A) 5'AAGCAA3' B) 5'AAGCTT3' C) 5'ACAACA3' D) 5'AGAGAG3' E) 5'AGCCGT3' F) 5'AATTAAC3'