Basic stains are more effective for bacterial staining than acidic dyes. Why? Which component on the cell wall of acid-fast bacteria prevents acid-alcohol from decolorizing protoplasm?
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A: Hans Christian Gram invented the Gram stain in 1884. (Gram,1884).
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A: Staining is a procedure that involves the application of colored chemicals called dyes to specimens.…
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Q: The bacterial cells in the image below were stained with a high concentration of carbolfuschin for…
A: Carbol fuchsin, carbolfuchsin, or carbol fuchsin is basically a mixture of "phenol" and basic…
Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…
A: Please follow step 2 for detailed explanation.
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Q: plain Why do acidic dyes not stain bacterial cells?
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Q: Why is mordant necessary for the staining of bacterial flagella? Answer in not more than 3…
A: In contrary to negative staining, which leaves the bacteria unstained against a dark backdrop,…
Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: How would the slide appear if the bacteria were stained with safranin, a basic red dye?
A: The appearance of bacteria, when stained with safranin, is to be determined.
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Q: Why do we use oil with microscope ?
A: ••In light microscopy, in order to increase the resolving power we use oil Immersion techniques
Q: What distinguishes Acid-Fast bacteria in terms of cell wall / coating and other characteristics?…
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Q: Principle/ technique used in Flagella Stain Method/ Preparation/ Procedure of Flagella Stain
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Q: bacteria require staining before microscopic examination?
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A: Answer :- Subsequent to trying different things with presenting cells to gold and mercury, Golgi…
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: what would be the medical significance of finding a positive endospore stain result from bacilli…
A: Endospores are thick walled structures found in the genus Bacillus and Clostridium.
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Q: Why does nigrosin not stain the bacteria?
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Q: Endospores can be seen as refractile structures in unstained cells and as colorless areas in…
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Q: do we need to stain microorganisms?
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Q: Are there any chemical differences between the cell walls of gram-positive and negative bacteria…
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Q: What type of chromophore is associated with a negative stain?
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Q: Why do acidic dyes not stain bacterial cells?
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Q: What are the principle and basic concepts of GRAM STAIN?
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Q: Why would specimens viewed with a compound microscope be thin and/or chemically cleared?
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Q: which statemts pertin to Gram stain, acid-fast stain, or spore stain. please group statements with…
A: Staining is an auxiliary method used to improve the transparency of the microscopic image used in…
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- Which type of stain would be the best choice for negative staining of most bacterial cells: a basic stain or an acidic stain? Explain your answer.Which type of stain would be the best choice for negative staining of most bacterial cells: a basic stain or an acidic stain?What stain would you use to differentiate between bean and ant cells? Explain and include your expected results.
- Why does endospore staining require heating? What chemical component of the endosporesprotects the cell from high temperature and chemical treatment?Simple Stain What is the difference between simple stain and complex stain? Describe the color of an S. aureus after performing simple stain with Safranin Red. Why? What do you conclude after performing simple stain and observing “Purple cells longer than they are wideWhat benefits do endospores provide the cells during staining procedures?
- Staining of cells is often performed to enhance images acquired by brightfield microscopy. Phase-contrast microscopy does not require cell staining. Why is this advantageous?The Gram staining (Ex. 3-6) procedure is exactly the same for Gram-positive and Gram-negative bacteria cells. So, why do the Gram-positive and Gram-negative cells end up looking very different after performing the Gram stain? (In your answer, explain the differences between the Gram-negative and Gram-positive bacteria cell wall structure and explain how the alcohol reacts differently with both the Gram-positive and Gram-negative bacteria cell walls.)which statemts pertin to Gram stain, acid-fast stain, or spore stain. please group statements with the correct term they pertain to Here are the descriptions: -does not require heat to be used in staining process -distinguishes between active metabolic cells and and dormant structures -a chemical is used as a mordant -differentiates cell with high lipid content in cell wall -uses carbol fuchsin, acid alcohol and methylene blue uses malachite green -differentitates cells based on thickness of peptidoglycan layer -result is purple and red/pink cells -important diagnostic tool in mycobacterium infections