What is the difference between simple stain and complex stain? Describe the color of an S. aureus after performing simple stain with Safranin Red. Why? What do you conclude after performing simple stain and observing “Purple cells longer than they are wide
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Simple Stain
- What is the difference between simple stain and complex stain?
- Describe the color of an S. aureus after performing simple stain with Safranin Red. Why?
- What do you conclude after performing simple stain and observing “Purple cells longer than they are wide
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- Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain procedure? Why or why not?What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Which type of stain would be the best choice for negative staining of most bacterial cells: a basic stain or an acidic stain?
- Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 1. Simple StainingBriefly discuss the difference between simple and differential staining? Which is better suited for common clinical microbiology laboratory applications?Is the flagellar stain a Positive or Negative Simple Stain protocol?
- Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 2. Gram StainingWhat color are Gram negative cells after decolorizing with ethanol? What is the best age for your culture when performing a Gram stain? Why? Why is Gram staining considered a differential staining process? What are the primary and counterstain? What is the purpose for using a counterstain? What are some reasons a Gram positive cell might appear Gram negative? What color would a Gram negative cell be if you forgot to wash with ethanol and why? What color would a Gram negative cell be if you forgot to stain it with safranin and why?What is a differential stain? why is it used?
- What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.Is it possible to perform double or triple staining using fluorescent dyes? Explain.What is the advantage of cryo-sectioning for antibody staining methods?