Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…
A:
Q: Why is heat necessary in spore staining?
A: BASIC INFORMATION SPORULATION It is a process during which spores are formed. We can find it in…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…
A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…
Q: What is gram staining and its purpose? Why is it only applicable in bacteria?
A: Introduction: Differential staining methods like Gram staining, acid-fast staining, endospore…
Q: Why can’t nitric acid stains be removed by washing?
A: Nitric acid: it has the chemical formula HNO3. It is a conjugate acid of nitrate and a protic…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes?
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: Please discuss why staining is important in visualizing microbes under the microscope. What…
A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: I need to discribe the "the colors of gram+ and gram (-) bacteria" in each independent step of the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: In your own words, explain how gram staining is done and how it works.
A: Gram staining is a popular method for distinguishing between two large groups of bacteria based on…
Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…
A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…
Q: Why is oil immersion necessary to see bacteria in the laboratory?
A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…
Q: Why are bacterial cells generally stained for microscopic viewing?
A: Staining bacterial cells prior to microscopic viewing is a routine process in most of microbiology…
Q: Give the principle of acid fast staining and gram staining.
A: Microorganisms are extremely small in size and hence cannot be visualized directly. Specialized…
Q: how can ultraviolet be used to control microbial growth. which specific UV can be used to control…
A: Ultraviolet (UV) - non-ionizing radiation. - penetration power is less. - It is a physical mutagen.
Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…
A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…
Q: In gram staining, if bacteria retain the primary stain after decolorizing agent treatment. Then the…
A: Answer: GRAM STAINING = It is the differential staining method to differentiate between gram…
Q: How is household bleach like a weapon against pathogenic bacteria?
A: pathogenic bacteria are those bacteria which can cause disease . they have to be eradicated .
Q: Both stained and live organisms are viewed using a microscope, but what is the purpose/benefit of…
A: Cell staining is a technique that is used in microscopy where certain chemicals can stick to…
Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…
A: Please follow step 2 for detailed explanation.
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: What is the purpose of using a biological stain when microscopically examining cellular components?
A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: With what kind of gram stain do you use heat fixation? What is the purpose of heat fixation? What…
A: Gram stain Gram staining is a method of staining which is used to classify the bacterial species…
Q: If you prepared a sample of a specimen for light microscopy, stained it with the Gram stain, and…
A: Bacteria are unicellular prokaryotic microorganisms (nucleus is not enclosed in a nuclear membrane)…
Q: Which stains are selective in microbiology?
A: INTRODUCTION Gram staining, acid fast staining, capsule staining and volutine granule staining are…
Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: List the steps in a Gram stain.
A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…
Q: Which step of the gram stain procedure is most critical?why?
A: Gram staining is one of the most common laboratories test to identify the different bacterial…
Q: Why are chemical stains required for visualizing cells and tissues with the basic light microscope?
A: Cell staining is a technique that is used to better visualize cells and cell components under a…
Q: Why must young cultures be used when doing a Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: Why is it important to center your object before moving to higher magnifications in a light…
A: A microscope is a crucial tool in the study of organisms that are very minute or to study the cells…
Q: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
A: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
Q: Why do we use oil with microscope ?
A: ••In light microscopy, in order to increase the resolving power we use oil Immersion techniques
Q: Why is it necessary to use contrasting stains while staining
A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…
Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…
A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…
Q: What is the advantage of gram staining over simple staining
A:
Q: Why is it incorrect to say that the GFP is a “staining” method?
A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…
Q: What is Gram staining?Describe step by step the procedure.
A: GRAM STAINING METHOD:- This is a differential staining that was developed by Dr. Hans Christian Gram…
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: why is gram staining important
A: Gram staining is a laboratory technique which is used to differentiate between different types of…
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: Which dye will not kill microorganisms if a wet preparation is made? Why?
A: Microorganisms are defined as organisms that cannot be viewed by the unaided eyes and are studied…
Q: agnification is required to observe microorganisms?
A: A microorganism, often known as a microbe, is a microscopic organism that can be a single cell or a…
Q: Why do acidic dyes not stain bacterial cells?
A: Stains may be divided into two classes based on the nature of their charged group Basic/ cationic…
Q: Why would specimens viewed with a compound microscope be thin and/or chemically cleared?
A: The human eye can see objects upto 0.1 millimeters. If the objects are smaller than this, the human…
Why do we need to stain microorganisms?
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