Below are gel electrophoresis results for initial and nested PCR of the Shrimp Plant. Use the results to answer these questions: a) Why does the Arabidopsis control generate two PCR bands, but the plasmid control only generates one PCR band? b) Did the negative control generate a PCR product? If so, what are the implications of this for the experiment? c) Would you use this nested PCR product from Shrimp Plant for cloning? Briefly explain your answer. 3 4 6. 7. 8 9 Lane 1 = 500 bp ladder Lane 2 = 8DNA shrimp initial PCR Lane 3 = 9DNA shrimp nested PCR Lane 4 = Arabidopsis initial PCR Lane 5 =Arabidopsis nested PCR Lane 6 = plasmid initial Lane 7 = plasmid nested %3D 2000 bp - 1500 bp - 1000 bp- Lane 8 = water initial Lane 9 = water nested 500 bp-

Question

Below are gel electrophoresis results for initial and nested PCR of the Shrimp Plant. Use the results to answer these questions:

a) Why does the Arabidopsis control generate two PCR bands, but the plasmid control only generates one PCR band?

b) Did the negative control generate a PCR product? If so, what are the implications of this for the experiment?        

c) Would you use this nested PCR product from Shrimp Plant for cloning?  Briefly explain your answer

Below are gel electrophoresis results for initial and nested PCR of the Shrimp Plant. Use the results to answer these questions:
a) Why does the Arabidopsis control generate two PCR bands, but the plasmid control only generates one PCR band?
b) Did the negative control generate a PCR product? If so, what are the implications of this for the experiment?
c) Would you use this nested PCR product from Shrimp Plant for cloning? Briefly explain your answer.
3
4
6.
7.
8 9
Lane 1 = 500 bp ladder
Lane 2 = 8DNA shrimp initial PCR
Lane 3 = 9DNA shrimp nested PCR
Lane 4 = Arabidopsis initial PCR
Lane 5 =Arabidopsis nested PCR
Lane 6 = plasmid initial
Lane 7 = plasmid nested
%3D
2000 bp -
1500 bp -
1000 bp-
Lane 8 = water initial
Lane 9 = water nested
500 bp-
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Transcribed Image Text

Below are gel electrophoresis results for initial and nested PCR of the Shrimp Plant. Use the results to answer these questions: a) Why does the Arabidopsis control generate two PCR bands, but the plasmid control only generates one PCR band? b) Did the negative control generate a PCR product? If so, what are the implications of this for the experiment? c) Would you use this nested PCR product from Shrimp Plant for cloning? Briefly explain your answer. 3 4 6. 7. 8 9 Lane 1 = 500 bp ladder Lane 2 = 8DNA shrimp initial PCR Lane 3 = 9DNA shrimp nested PCR Lane 4 = Arabidopsis initial PCR Lane 5 =Arabidopsis nested PCR Lane 6 = plasmid initial Lane 7 = plasmid nested %3D 2000 bp - 1500 bp - 1000 bp- Lane 8 = water initial Lane 9 = water nested 500 bp-