Below is a chromatogram of a mixture of phenolic pollutants obtained from the analysis of a river water sample using HPLC reversed-phase chromatography. Based on this chromatogram, answer the following questions. (1) (ii) (iii) (iv) 0.01 TO 21. 1.0 2.030 - 2.0 B с 51712. 2.521 1187 02. 3.2 What is meant by 'retention time'? Calculate the capacity factor, k, for compounds 1 and 2. Calculate the selectivity factor, a, for compounds 1 and 2. Suggest two parameters (or factors) that may affect the selectivity factor, a, for the two compounds?
Below is a chromatogram of a mixture of phenolic pollutants obtained from the analysis of a river water sample using HPLC reversed-phase chromatography. Based on this chromatogram, answer the following questions. (1) (ii) (iii) (iv) 0.01 TO 21. 1.0 2.030 - 2.0 B с 51712. 2.521 1187 02. 3.2 What is meant by 'retention time'? Calculate the capacity factor, k, for compounds 1 and 2. Calculate the selectivity factor, a, for compounds 1 and 2. Suggest two parameters (or factors) that may affect the selectivity factor, a, for the two compounds?
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.14QAP
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