Below is a chromatogram of a mixture of phenolic pollutants obtained from the analysis of a river water sample using HPLC reversed-phase chromatography. Based on this chromatogram, answer the following questions. (1) (ii) (iii) (iv) 0.01 TO 21. 1.0 2.030 - 2.0 B с 51712. 2.521 1187 02. 3.2 What is meant by 'retention time'? Calculate the capacity factor, k, for compounds 1 and 2. Calculate the selectivity factor, a, for compounds 1 and 2. Suggest two parameters (or factors) that may affect the selectivity factor, a, for the two compounds?

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.14QAP
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Below is a chromatogram of a mixture of phenolic pollutants obtained from the analysis of a
river water sample using HPLC reversed-phase chromatography. Based on this
chromatogram, answer the following questions.
(i)
(ii)
(iii)
(iv)
0.01
TO
0.8
1.2
0".
BEO
- 2.0
1757
$122.
2.8
3.2
What is meant by 'retention time'?
Calculate the capacity factor, k, for compounds 1 and 2.
Calculate the selectivity factor, a, for compounds 1 and 2.
Suggest two parameters (or factors) that may affect the selectivity factor, a, for the
two compounds?
Transcribed Image Text:Below is a chromatogram of a mixture of phenolic pollutants obtained from the analysis of a river water sample using HPLC reversed-phase chromatography. Based on this chromatogram, answer the following questions. (i) (ii) (iii) (iv) 0.01 TO 0.8 1.2 0". BEO - 2.0 1757 $122. 2.8 3.2 What is meant by 'retention time'? Calculate the capacity factor, k, for compounds 1 and 2. Calculate the selectivity factor, a, for compounds 1 and 2. Suggest two parameters (or factors) that may affect the selectivity factor, a, for the two compounds?
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