Help! Of not leaving a stain on a smear long enough (under-staining)?It would under-Stain. Ihe cells will be difficut toSee.5 Choose a coccus and a bacillus from the organisms you observed and calculate their surface-to-volume ratios.Consider the coccus to be a perfect sphere and the bacillus to be a cylinder. Use the equations supplied.Cell MorphologySurface AreaVolumeСосcusSA = 4Tr2v =D43BacillusSA = 2mrh + 2Tr? = md(r+h)%3DV = Tr?h%3D%3Dr = radius, d = diameter, h = height, T = 3.14Surface-to-Volume Ratio of Sample CellsCellSurface AreaVolumeOrganismMorphology(um²)(um³)Surface-to-Volume Ratio4 Consider a coccus and a rod of equal volume.a. Which is more likely to survive in a dry environment? Explain your answer.

Given surface are of sphere = 4πr2 . volume of sphere = 4/3πr3 . Given surface are of cylinder…

Answered: Of not leaving a stain on a smear long…

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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An acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following couid explain why only blue cells were present?
Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.
Which parts of the modern microscope do you think were absent from Leeuwenhoek’s microscope? Why is it necessary to calibrate the ocular micrometer with each objective? When measuring dimensions of microorganisms, what slide preparation should be used and why? Complete the following for each objective in your microscope: _____ Ocular micrometer divisions = _________ stage micrometer divisions _____ Ocular micrometer divisions = _________1 stage micrometer division = _____mm Microscopic Measurements 1 Arrange the following items by using numbers (smallest) to 8 (largest). ______ AIDS virus ______ rickettsia ______ worm ______ white blood cell (WBC) ______ amoeba ______ protein ______ staphylococci…
Using an appropriate lens and oil immersion, what would be a microscope's maximum resolution (in micrometres) that could use a wavelength of 350 nm (assuming that such a device exists)? What would be the surface-to-volume ratio for the coccus-shaped bacterium twice the size of the maximum resolution you calculated? Why is the surface-to-volume ratio an important biological factor. Explain. Show all of your calculations.
If 5X instead of 10X oculars were used with the same objectives now on your microscope, what magnifications would be achieved? Explain your answer.
PARTS OF THE MICROSCOPE. Match Column A with Columns B and C. Example: 1). A, C
Two monocular compound microscopes properly spacedand aligned describe the__________ microscope.
The Gram staining (Ex. 3-6) procedure is exactly the same for Gram-positive and Gram-negative bacteria cells. So, why do the Gram-positive and Gram-negative cells end up looking very different after performing the Gram stain? (In your answer, explain the differences between the Gram-negative and Gram-positive bacteria cell wall structure and explain how the alcohol reacts differently with both the Gram-positive and Gram-negative bacteria cell walls.)
Using the above diagram, point out all the different parts of the microscope in a numeric fashion.
Give typing answer with explanation and conclusion 1.During the endospore staining method we use the boiling of water on a tripod technique. Why?
Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. At 100X you are unable to see the diatom. Explain why. Explain what correction (s) should be made to allow you to see the diatom using the 100X lens. After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred.
Can someone answer and explain the following? What are the basic components of a Fluorescence Microscope and what are the functions of each? Are there any parts that you can remove without compromising accuracy and utility of the equipment? Can you suggest additional components to improve the equipment?

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