- Which parts of the modern microscope do you think were absent from Leeuwenhoek’s microscope?
- Why is it necessary to calibrate the ocular micrometer with each objective?
- When measuring dimensions of microorganisms, what slide preparation should be used and why?
- Complete the following for each objective in your microscope:
_____ Ocular micrometer divisions = _________ stage micrometer divisions
_____ Ocular micrometer divisions = _________1 stage micrometer division = _____mm
Microscopic Measurements 1
- Arrange the following items by using numbers (smallest) to 8 (largest).
______ AIDS virus ______ rickettsia
______ worm ______ white blood cell (WBC)
______ amoeba ______ protein
______ staphylococci ______ atom
- Complete the following table on units’ measurement.
|
Unit |
Shorthand |
Value |
|
1 centimeter |
1 centimeter |
10-2 meter |
|
1 millimeter |
1 millimeter |
|
|
|
|
10-6 meter |
|
1 nanometer |
1 nanometer |
10-9 meter |
|
1 angstrom |
1 angstrom |
10-10 meter |
|
10 micrometer |
10 micrometer |
|
|
|
|
|
- Calibrate each objective of your microscope using an ocular and stage micrometer and complete the table below.
|
|
|
µm |
mm |
|
LPO (10X) |
1 ocular space |
|
|
|
HPO (40X) |
1 ocular space |
|
|
|
OIO (100X) |
1 ocular space |
|
|
Note: These numerical values hold only for the specific objective-ocular lens combination used and may vary with different microscopes.
- Answer the following:
- a)25 spaces on ocular = 7 spaces on the stage
Thus, one ocular space = _______ and one ocular space = _____ mm or ____ µm
- b) 17 spaces on the ocular = 11 spaces on the stage
Thus, one ocular space = ______ and one ocular space = _____ mm or____µm
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