You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and counted 67 cfu. What was the cell density of your bacterial culture (in cfu/ml? How many cells did you have in total if the volume of your culture was 50ml? Round to a whole number, do not write in scientific notation. The cell density of my bacterial culture was cfu/ml. The total number of cells was
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- You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell density of this culture?A 2x10-6 dilution of your bacterial culture yielded 10 colonies. What was the cell density (incells/ml) of your bacterial culture?The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?
- The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per mL. For your experiment, you first dilute the culture 100 fold. Assuming that there is no lab phase and that the cells remain in exponential growth the entire time, what is the cell density (cells/mL) after 10 hours?Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume? suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.
- "If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many colony forming units (CFU) will be reported?" 70 700 7000 70000 700000You have spread 0.1ml of a 1x10-8 diluted bacterial culture sample on a Petridish and counted35 colonies. What was the cell density of your original culture (in cells/ml)? How many cellsdid you have in 100ml of that culture? DON’T FORGET TO ROUND!) If you counted 500 CFU’s in the petri dish for number 6 and 5000 CFU’s in petri dish for number 7, what would be the most justifiable explanation for this? 19) If the original sample’s concentration was 12 bacterial cells, what do you expect the count to be?
- You perform a set of serial dilutions using 99-mL water blanks (successive 1-mL dilutions into blanks A, B, and C, respectively). Following spread plating and incubation of a 0.1 mL subsample from Blank C, you count 66 colonies on the plate. How many colony-forming units are in the original broth culture?If 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of the original cultureYou aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is the dilution factor?