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- What is the complement of 5’ -AGGTCGTAC-3’ ? 5’ – GCTAGTCAG-3’ 3’- AGGTCGAGT-5’ 3’- GAACTACGT-5’ 5’-TCCAGCATG-3’ 3’- TCCAGCATG-5’Decoding a DNA Message 2. AAT CTC CGA GCT TTG TAG TTA CCC ATT TAG AGT ATC TAG TTG TGT CTC GCT CTC AGG TGC TAT TTG CCT ACTWILD-TYPE MC1R GENE (LIGHT COAT-COLOR PHENOTYPE DNA TTG AGG TGG GCG TGT CCG CAA GGA mRNA Amino Acid
- WILD-TYPE MC1R GENE (LIGHT COAT-COLOR PHENOTYPE) DNA CGG GAC CGG TGG GCC CAC TGA CAC mRNA Amino AcidWhich of the following is NOT a TSE? CJD kuru Alzheimer’s BSE Which of the following is NOT a process in immunoglobulin differentiation? VDJ recombination Disulfide bond formation Isotype switching Splicing of the primary transcriptCHOICES: Isoniazid Rifampicin Ethambutol None of the Choices Causes more adverse effects to slow acetylators Causes optic neuritis Causes orange secretions Inhibits transcription process in mycobacteria Binds to bacterial DNA gyrase & topoisomerase
- 3. The sequence below used the sequence in “1” and encountered a point mutation: TAC-CTA-CGT-AGG-GTT-TAC-ACC-ACA-AGC-CGT-TGG- AAT -GTG-CCA-AAG-TTG-CAG-TGA-AGG- TGA-TGC-TAG-TGA-AAG-CTA-GAT-AGG-GCT-TAT-ATG-AGA-TTT-CGC-CCC-CGA-TAC-GAT-TTG- CTG-AAT-AGA-CTC-GAT-GAA-TCG-CGC-GAT-GTT-ACT a. Determine the mRNA produced by transcription: b. Determine the amino acid sequence after translation (USE “-“ TO SEPARATE AMINO ACIDS, NO SPACES): c. What is the type of point mutation that occurred? A. Nonsense B. Silent C. Missense3. The sequence below used the sequence in “1” and encountered a point mutation: TAC-CTA-CGT-AGG-GTT-TAC-ACC-ACA-AGC-CGT-TGG- AAT -GTG-CCA-AAG-TTG-CAG-TGA-AGG- TGA-TGC-TAG-TGA-AAG-CTA-GAT-AGG-GCT-TAT-ATG-AGA-TTT-CGC-CCC-CGA-TAC-GAT-TTG- CTG-AAT-AGA-CTC-GAT-GAA-TCG-CGC-GAT-GTT-ACT a. Determine the mRNA produced by transcription: b. Determine the amino acid sequence after translation (USE “-“ TO SEPARATE AMINO ACIDS, NO SPACES): c. What is the type of point mutation that occurred? 4. The sequence below used the sequence in “1” and encountered a type of frameshift mutation: TAC-CTA-CGT-AGG-GTT-TAC-ACC-ACA-AGC-CGT-TGG-AAC-GTG-CCA-AAG-TTG-CAG-TGA-AGG- TGA-TGC-TAG-TGA-AAG-CTA-GAT-AGG-GCT-TAT-ATG-AGA-TTT-CGC-CCC- CGT -ACG-ATT-TGC- TGA-ATA-GAC-TCG-ATG-AAT-CGC-GCG-ATG-TTA-CT a.Determine the mRNA produced by transcription: b.Determine the amino acid sequence after translation (USE “-“ TO SEPARATE AMINO ACIDS, NO SPACES): c.What is the type of point mutation that occurred? a. Silent mutation…How long would the peptide be that is translated from this MILNA sequence: 5-AUGGGCUACCGA-3? a. 0 b. 2 c. 3 d. 4
- An experiment was performed in mice. Wild-type bone marrow is used to reconstitute lethally irradiated Cr2-/- mice (wt®Cr2-/-), or vice versa (Cr2-/-®wt). As controls, Cr2-/- bone marrow is used to reconstitute Cr2-/- recipients (Cr2-/-®Cr2-/-), and wild-type bone marrow used to reconstitute wild-type recipients (wt®wt). These mice are then inoculated with the HSV-rd virus at 106 PFU, once at day 0 and then a second time at day 28, and the anti-HSV IgG responses are measured every 7 days, as shown in figure below. a) From the data shown above, on which cell type is the expression of the complement receptor most important for humoral immunity? b) For each of the cell types expressing the complement receptor encoded by Cr2, what is the explanation for their importance in humoral immunity to HSV inoculation?My professor instructed me to make a power point presentation on this topic" Prenatal Molecular Testing ". I have total 4 members in my group, Now can you divide this topic into 4 sub topic or parts, which we can work? Please divide the topic into 4 parts, i will rate you positive if you do so. Thank you. ?Decoding a DNA Message 1. CGA ACT AGA TAA CCC TTA TAG AAA TAT ACA CGC TTG TGA AAC ATA ATC CGG CTG CAA CGT TTA ACA CTC CTA ATT AGG ACA TAA CTC TTG ACA CTT ACT TAG AGA ACT CTC CGC TCA TAG GAG ATA ATT TAC TAA AGA TGA CGG TTT CTT TTG ACT CGA AGC ATT TAC CGC CCC TAG ATC 2. AAT CTC CGA GCT TTG TAG TTA CCC ATT TAG AGT ATC TAG TTG TGT CTC GCT CTC AGG TGC TAT TTG CCT ACT