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WILD-TYPE MC1R GENE (LIGHT COAT-COLOR
|
DNA |
CGG |
GAC |
CGG |
TGG |
GCC |
CAC |
TGA |
CAC |
|
mRNA |
||||||||
|
Amino Acid |
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- WILD-TYPE MC1R GENE (LIGHT COAT-COLOR PHENOTYPE DNA TTG AGG TGG GCG TGT CCG CAA GGA mRNA Amino AcidWILD-TYPE MC1R GENE (LIGHT COAT-COLOR PHENOTYPE) DNA GTG TAC GAA CGT mRNA Amino Acid#3 HaelII --- 5’ CC ↓ GG 3’ 5’ ACGCCGGCCGTATTAT CCGGATCCGCCG CCGGCTGTCCCGGATCA 3’ 3’ TGCGGCCGGCATAATAGGCCTAGGCGGCGGCCGACAGGGCCTAGT 5’ Restriction enzyme: Recognition sequence: Number of pieces of DNA: Type of cut:
- #1 HindII --- 5’ GTC ↓ GAC 3’ 5’ ACGACGTAGTCGACTTATTAT GTCGACCCGCCGCGTGTCGACCATCA 3’ 3’ TGCTGCATCAGCTGAATAATACAGCTGGGCGGCGCACAGCTGGTAGT 5’ Restriction enzyme: Recognition sequence: Number of pieces of DNA: Type of cut:somatic cells and CRISPR Cas9#4 BamI --- 5’ CCTAG ↓G 3’ 5’ ACGCCTAGGACGTATTATCCTAGGTAT CCGCCGCCGT CATCA 3’ 3’ TGCGGATCCTGCATAATAGGATCCATAGGCGGCGGCAGTAGT 5’ Restriction enzyme: Recognition sequence: Number of pieces of DNA: Type of cut:
- #2 EcoRI --- 5’ G ↓AATTC 3’ 5’ ACG ACGTATTAGAATTCTTA TCCGCCGCCGGAATTCT CATCA 3’ 3’ TGC TGCATAATCTTAAGAATAGGCGGCGGCCTTAAGAGTAGT 5’ Restriction enzyme: Recognition sequence: Number of pieces of DNA: Type of cut:GENE TABLE 1: WILD-TYPE MC1R GENE (LIGHT COAT-COLOR PHENOTYPE) DNA GAA CAG GTG GTT CCA AAG GCT GAG mRNA Amino AcidPrimer1: seq1 atatatatccccccatcactgggggg Primer 2: seq2 tatatactagggtacgtatgcccccc
