Answered: Both the isolation buffer and assay…

Both the isolation buffer and assay buffer that was used in isolation of mitochondria contained 0.3M mannitol. What was the purpose of including mannitol at this concentration?

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter6: The Behavior Of Proteins: Enzymes

Section: Chapter Questions

Problem 28RE: MATHEMATICAL The enzyme -methylaspartase catalyzes the deamination of -methylaspartate [V. Williams...

See similar textbooks

Related questions

Q: Using island biogeography theory, on which island would you expect to find a greater number of…

A: The idea of island biogeography was proposed by Wilson and MacArthur in 1967.

Q: Why should changing the genome of human embryos ( human germline gene editing) not be allowed?…

A: CRISPR is a acronym for clustered regularly interspaced short palindromic repeats. It is a gene…

Q: Dizygotic twins are no more or less genetically similar than are other pairs of ________________.

A: Twins are named so because some of their completely identical features but there are certain…

Q: when it rains why do we see more worms out? Use the scientific method steps to describe why we see…

A: Earthworms are a sign of good soil. These are necessary for the breakdown of organic debris (such as…

Q: when it rains why do we see more worms out?

A: Animal kingdom is a higher kingdom that comprises of eukaryotic organisms. Animal kingdom is…

Q: Multiple Choice Question In an insect sensillum: a. the Semper cell forms the socket. b. the…

A: Introduction:- The sensillum of an arthropod has a sensory organ that protruding from the cuticle…

Q: Physician Edward H. Clarke published a treatise opposing higher education for women (Clarke, 1873).…

A: Dr. Edward H. Clarke's in 1873 had to face severe negative reactions from their opponents when he…

Q: How early man could easily perceive the difference between inanimate matter and living organisms

A: Traditionally, when we try to define life, we look for specific traits that living things exhibit.…

Q: mitosis

A: Mitosis: It is defined as the process of cell duplication where one cell divides into two…

Q: Ture or False: (T/F) A bee would detect the odor of the flowers using trichoid sensilla located on…

A: True True False

Q: Crossing over is an event occurring during metaphase II. True False

A: Crossing over is a cellular process that happens during meiosis a special type of cell division that…

Q: Matter is continually recycled between abiotic and biotic components of Earth's ecosystem in…

A: The nitrogen cycle is the biogeochemical cycle by which nitrogen is converted into multiple usable…

Q: 20. Record the type of test used to test for the following organic compounds and what result…

A: To determine whether organic chemicals are present, straightforward chemical tests can be performed…

Q: If a liver cell in an insect contains 24 chromosomes, how many chromosomes are found in its sperm?…

A: Gametogenesis is a specific interaction through meiosis where gametes are produced. The gametes…

Q: Barn Owls produce 2 pellets per day. Assume each pellet contains 80g of biomass. Also assume that…

A: Given Barn owl produces 2 pellets per day. Each pellet has 80 gram of biomass. 40 gram vo le can…

Q: 11. How are metaphase 12. If cells in the process of dividing are subjected to colchicine, a drug…

Q: Selection confers a reproductive advantage to individuals based on their adaptations, and therefore…

A: When reproduction takes place, the two types of gametes are coming together and form a zygote which…

Q: Receptor 1 Receptor 2 Drug A 8.0 7.8 Receptor 5.2 3 Drug B 8.5 PA value 6.4 Drug C 3.1 6.5 10.5 6.5…

A: The transfer of medicine from the administration to the bloodstream is known as absorption. The drug…

Q: Discuss salient features of protozoan

A: Protozoans are the tiny, unicellular and microscopic organisms that contains complex structures with…

Q: Which hormone and condition are mismatched? a. growth hormone-acromegaly b. thyroxine-goiter c.…

A: We know that the Endocrine gland is a gland that liberates its secretion into the blood rather than…

Q: airways clean. ribosomes cell walls cilia vacuoles microvilli present on the cells of our trachea…

A: Explain;- Cilia line the trachea as well, removing foreign particles and fluids from the airway and…

Q: Homozyogous wild type male Drosophila PPQQRRSSTTUUVV (all linked) are irradiated to induce the…

A: Genes are small units of heredity, a different version of the same gene is known as its allele.…

Q: What can be done at home to reduce the amount of bacteria on food?

A: Bacteria These are single-celled prokaryotic organisms that are present almost everywhere. Some…

Q: why is it more advantageous for the cell to have its DNA fused to the cell wall as opposed to…

A: DNA is the nucleic acid present in majority of the organisms,ranging from prokaryotes to eukaryotes.

Q: Phytonadione, also known as Vitamin K1, is required by the body for the modification of certain…

Q: Discuss how global warming might make it harder for the wind/currents to mix the different ocean…

A: Methane gas is produced by microorganisms that break down plant and animal material. This is a…

Q: An individual with an energy expenditure of 30 L of O2/mile and an RER of 0.2 would have to run how…

A: There is a non-linear courting among strolling pace and price of calorie burn. Essentially what this…

Q: Explain in your own words what is the environmental significance of pH.

A: pH is a very important tool for assessing the acidity or alkalinity of a substances.

Q: where are the bacteria that did not take up the plasmid during the transformation

A: Introduction : The technique of horizontal gene transfer known as bacterial transformation allows…

Q: Discuss physiology of protozoan

A: Protozoans are the unicellular eukaryotic organisms that can be considered as free living or…

Q: The hard, often massive rock-like structures of stony corals are actually exoskeletons comprised of…

A: Anthozoa is a class of marine invertebrates which includes variety of animals like stony corals, sea…

Q: ►Describe the effects of enzyme concentration on enzyme activity

A: Every step in the biological process depends on enzymes. They catalyse the sequential reactions…

Q: The difference between asexual and sexual reproduction is that O A. asexual reproduction occurs only…

A: The generation of descendants can be referred to as reproduction. Asexual reproduction is one of the…

Q: Vaccine mandates are often justified as necessary to protect public health (and do the most good for…

A: In order to understand what is ethically wrong or right, ethics explores the intellectual…

Q: What are three functions of the cell nucleus? What are two functions of a plant cell vacuole?

A: Introduction: Nucleus: The largest and most noticeable organelle inside the cell is the nucleus. The…

Q: Types of life-threatening arrythmias in adults

A: Arrhythmias is generally due to very low potassium or magnesium or it is associated with…

Q: Which Scyphozoan life stage performs sexual reproduction? Gemmule Planula Scyphystoma Medusa Ephyra

A: The members of Class Scyphozoan are marine and mainly consist of jelly fishes.The life cycle of…

Q: Which of the following statements are is true about the distribution of hermatypic corals? They…

A: Corals are created by organisms from the Phylum Coelenterata Coral reef is a habitat to many other…

Q: a. Give the three-letter and one-letter names of the amino acids in each oligopeptide starting from…

A: A peptide (peptide bond) is an amide linkage formed by the reaction between the α-carboxyl group of…

Q: Couple 2 PKU heterozygote (carrier) PKU homozygote Couple 1 B. Can couple 3 have a child that has…

A: Since couple 1 who are not affected have affected children - one male and one female, the mode of…

Q: Do you think that most plant and animal cells are similar to does we observed today?

A: Since you have asked multiple questions we will answer the first question for you. If you want any…

Q: Discuss the importance of international agreements in the management of biomedical waste

A: Biomedical waste: It is any waste that is generated during the diagnosis, treatment, and…

Q: endocytosis pinocytosis symbiosis exocytosis keratosis A macrophage is engulfing bacteria through…

A: Introduction :- Macrophages are immune cells that reside or are infiltrated inside tissues and are…

Q: Briefly explain how multi factorial variables in the environment act concurrently

A: A set of observations, each described by several different sets of variables, is subjected to…

Q: What do you understand by cis & trans regulations? Describe.

A: Cis and Trans are the regulatory element which play a important role in gene expression.Promotres,…

Q: Draw the two dipeptides formed from each pair of amino acids. a. tyrosine and lysine b. threonine…

A: Amino acids are the structural unit of proteins. Amino acids (same or different) join with one…

Q: An E. coli colony grew on minimal medium supplemented with arginine and leucine. However, bacteria…

A: Introduction :- The coliform bacterium Escherichia coli, usually known as E. coli, belongs to the…

Q: Compare and contrast between intrachromosomal translocation and unequal crossing over

A: The study of genomes' structure, functioning, evolution, sequencing, and editing is the emphasis of…

Q: Fill-in (complete each statement with the correct term) 1. The are three passages, each forming a…

A: Human anatomy is the scientific study of mature human morphology. There are two categories within…

Q: Inhibitory interneurons associated with the reflex arc are turned on by glutamate. Group of answer…

A: The brain is the primary organ of the nervous system, accountable for receiving and interpreting…

Concept explainers

Food Microbiology

The subdivision of microbiology that deals with the interaction between microbes and food particles are called food microbiology. This scientific study incorporates both beneficial and harmful microbes and their effect on the food production, food storage, and health of human beings.

Question

50% 110 min 47 secs
Both the isolation buffer and assay buffer that was used in isolation of mitochondria contained 0.3M mannitol. What was the purpose of including
mannitol at this concentration?
223 ¶¶ 2 PP GƏ
Typed answer only and also detailed
explanation.
by ProPros
Create a Q

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Answer

VIEW

Explanation

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

14) Calculate K’eq and ΔG’0 for the following: A 0.1 M solution of glucose 1-phosphate at 25oC is incubated with a catalytic amount of phosphoglucomutase, the glucose 1-phosphate is transformed to glucose 6-phosphate. At equilibrium, the concentrations of the reaction components are Glucose 1-phosphate (4.5 X 10-3 M) and Glucose 6-phosphate (9.6 X 10-2 M).
5 900uL of lysozyme solution was added with 100uL of Fraction X into a cuvette. In a kinetic measurement using spectrophotometer, the Abs/min value for lysozyme cell wall hydrolysis activity was -0.040. The settings were at 450nm, 3 second intervals. Calculate the concentration of lysozyme activity that is present, in units of abs/min/mL. If you could show and explain how to calculate, that would be amazing
Please help me answer and explain these questions: 1. Compare the abundance of A280 chromophores (W, Y, and C) to Bradford sites (loosely R and K) in BSA. 2. We used a protocol that corrects the path length in each well and normalizes the absorbance to the value that would be obtained if the path length were 1 cm (the standard path length). Using this observation and your A280 calibration data, what is your measured e280, for BSA?
Q. You have now carried out the Somogyi Nelson determination of reducing sugars and measured the absorption spectra of Tube 9. What is the optimum wavelength you should use to monitor the Somogyi Nelson Assay why? Table 1._SOMOGYI-NELSON DTERMENATION OF REDUCING SUGARS Tube 1 2 3 4 5 6 7 8 9 10 D-Glucose (160 µg/mL), mL 0 0 0.125 0.125 0.25 0.25 0.375 0.375 0.5 0.5 H2O, mL 0.5 0.5 0.375 0.375 0.25 0.25 0.125 0.125 0 0 D-Galactose (160 µg/mL), mL - - - - - - - - - - D-Arabinose (160 µg/mL), mL - - - - - - - - - - Glucose-Galactose Mixture, mL - - - - - - - - - - Absorbance (710 nm) 0.049 0.051 0.138 0.151 0.271 0.26 0.382 0.395 0.454 0.444 Absorbance - blank 0 0 0.088 0.101 0.221 0.21 0.332 0.345 0.404 0.394 Final [Reducing sugar]…
Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?
12. When a person is determining the concentration of a sample, they will frequently repeat the samemeasurement of a sample by preparing three or more identical tubes (i.e. make replicates).a) Although you did not set up replicates today, what would be the purpose of setting upreplicates?b) If you had tested three replicates of test tube #2 in the determination of glucoseconcentration, what would you do with the data from the three replicates?
11) Using the data above, construct Lineweaver-Burk plots. First, graph the inverse of the reaction velocity (rate of reaction) data in column II versus the inverse of the methanol concentration in column I. Then, on the same graph, plot the inverse of the reaction velocity (rate of reaction) data in column III versus the inverse of the methanol concentration in column I
All questions a) What is the hypothesis of Griffith’s experiment? b) Why did mouse No. 3 survived? c) Why did mouse No. 4 died? d) If Griffith had injected a fifth mouse with a combination of heat-killed R strain bacteria and heat-killed S strain bacteria, would the mouse have died? Give your reason.
2c which is altered by a catalyst such as an enzyme, the net free energy change of the reaction or the activation energy of the reaction?
Ex. 5-4 Catalase 1) In the Catalase test (5-4), if bacteria was added to hydrogen peroxide on the slide, it might produce a false result. 2) Explain why adding bacteria to the hydrogen peroxide on a slide rather than adding the hydrogen peroxide to the bacteria on the slide might give a false result? 3) Would the false result be a false positive or would the false result be a false negative?
After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.
1. How can ADP-ATP translocator be purified through affinity chromatography? Please write a step by step general detailed procedure. 2. What does the 2 cycles within the Q cycle of Complex 3 in terms of making ATP do?