Q. You have now carried out the Somogyi Nelson determination of reducing sugars and measured the absorption spectra of Tube 9. What is the optimum wavelength you should use to monitor the Somogyi Nelson Assay why? Table 1._SOMOGYI-NELSON DTERMENATION OF REDUCING SUGARS Tube 1 2 3 4 5 6 7 8 9 10 D-Glucose (160 µg/mL), mL 0 0 0.125 0.125 0.25 0.25 0.375 0.375 0.5 0.5 H2O, mL 0.5 0.5 0.375 0.375 0.25 0.25 0.125 0.125 0 0 D-Galactose (160 µg/mL), mL - - - - - - - - - - D-Arabinose (160 µg/mL), mL - - - - - - - - - - Glucose-Galactose Mixture, mL - - - - - - - - - - Absorbance (710 nm) 0.049 0.051 0.138 0.151 0.271 0.26 0.382 0.395 0.454 0.444 Absorbance - blank 0 0 0.088 0.101 0.221 0.21 0.332 0.345 0.404 0.394 Final [Reducing sugar] (µg/mL) 0 0 40 40 80 80 120 120 160 160 Tube 11 12 13 14 15 16 D-Glucose (160 µg/mL), mL - - - - - - H2O, mL - - - - - - D-Galactose (160 µg/mL), mL 0.5 0.5 - - - - D-Arabinose (160 µg/mL), mL - - 0.5 0.5 - - Glucose-Galactose Mixture, mL - - - - 0.5 0.5 Absorbance (710 nm) 0.461 0.468 0.426 0.416 0.321 0.312 Absorbance - blank 0.411 0.418 0.376 0.366 0.271 0.262 Final [Reducing sugar] (µg/mL) - - - - - -
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
Q. You have now carried out the Somogyi Nelson determination of reducing sugars and measured the absorption spectra of Tube 9. What is the optimum wavelength you should use to monitor the Somogyi Nelson Assay why?
Table 1._SOMOGYI-NELSON DTERMENATION OF REDUCING SUGARS
|
Tube |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
|
D-Glucose (160 µg/mL), mL |
0 |
0 |
0.125 |
0.125 |
0.25 |
0.25 |
0.375 |
0.375 |
0.5 |
0.5 |
|
H2O, mL |
0.5 |
0.5 |
0.375 |
0.375 |
0.25 |
0.25 |
0.125 |
0.125 |
0 |
0 |
|
D-Galactose (160 µg/mL), mL |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
D-Arabinose (160 µg/mL), mL |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
Glucose-Galactose Mixture, mL |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
Absorbance (710 nm) |
0.049 |
0.051 |
0.138 |
0.151 |
0.271 |
0.26 |
0.382 |
0.395 |
0.454 |
0.444 |
|
Absorbance - blank |
0 |
0 |
0.088 |
0.101 |
0.221 |
0.21 |
0.332 |
0.345 |
0.404 |
0.394 |
|
Final [Reducing sugar] (µg/mL) |
0 |
0 |
40 |
40 |
80 |
80 |
120 |
120 |
160 |
160 |
|
Tube |
11 |
12 |
13 |
14 |
15 |
16 |
|
D-Glucose (160 µg/mL), mL |
- |
- |
- |
- |
- |
- |
|
H2O, mL |
- |
- |
- |
- |
- |
- |
|
D-Galactose (160 µg/mL), mL |
0.5 |
0.5 |
- |
- |
- |
- |
|
D-Arabinose (160 µg/mL), mL |
- |
- |
0.5 |
0.5 |
- |
- |
|
Glucose-Galactose Mixture, mL |
- |
- |
- |
- |
0.5 |
0.5 |
|
Absorbance (710 nm) |
0.461 |
0.468 |
0.426 |
0.416 |
0.321 |
0.312 |
|
Absorbance - blank |
0.411 |
0.418 |
0.376 |
0.366 |
0.271 |
0.262 |
|
Final [Reducing sugar] (µg/mL) |
- |
- |
- |
- |
- |
- |
![Table 1._SOMOGYI-NELSON DTERMENATION OF REDUCING SUGARS
Tube
1
2
3
4
7
8
9
10
D-Glucose (160 µg/mL), mL
0.125
0.125
0.25
0.25
0.375
0.375
0.5
0.5
H20, mL
0.5
0.5
0.375
0.375
0.25
0.25
0.125
0.125
D-Galactose (160 ug/mL),
mL
D-Arabinose (160 ug/mL),
mL
Glucose-Galactose Mixture,
mL
Absorbance (710 nm)
0.049
0.051
0.138
0.151
0.271
0.26
0.382
0.395
0.454
0.444
Absorbance - blank
0.088
0.101
0.221
0.21
0.332
0.345
0.404
0.394
Final [Reducing sugar]
40
40
80
80
120
120
(ug/mL)
160
160
Tube
11
12
13
14
15
16
D-Glucose (160 µg/mL), mL
H20, mL
D-Galactose (160 µg/mL), mL
0.5
0.5
D-Arabinose (160 µg/mL), mL
0.5
0.5
Glucose-Galactose Mixture, mL
0.5
0.5
Absorbance (710 nm)
0.461
0.468
0.426
0.416
0.321
0.312
Absorbance - blank
0.411
0.418
0.376
0.366
0.271
0.262
Final [Reducing sugar] (µg/mL)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc9754965-9702-4ddc-bf45-5a2acc6d5d62%2F685084c4-b720-44d3-85df-9293f214366a%2Fi6ecvq_processed.jpeg&w=3840&q=75)
Step by step
Solved in 2 steps
