Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. What to do? Calculating the average absorbance of the standards Subtracting the absorbance of the blank (the sample with 0 g/ml) from the absorbance of the other samples Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.319

Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. What to do? Calculating the average absorbance of the standards Subtracting the absorbance of the blank (the sample with 0 g/ml) from the absorbance of the other samples Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.319

Name: Bradford AssayFill in the average A595 and A595 of sample minus A595 o
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Description: Bradford AssayFill in the average A595 and A595 of sample minus A595 of blank. What to do?Calculating the average absorbance of the standardsSubtracting the absorbance of the blank (the sample with 0 g/ml) from the absorbance of the other samplesConc

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.3: Principles Of Chemistry Instrumentation

Problem 1CT

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Concentration (micrograms/ml)	A595 - trial 1	A595 - trial 2	A595 - trial 3	average A595	A595 of sample minus A595 of blank
0	1.501	1.446	1.447
2	1.624	1.558	1.559
5	1.731	1.749	1.712
10	1.901	1.838	1.892
15	2.161	2.108	2.228
18	2.231	2.277	2.319