Briefly describe the importance of origin of replication in a cosmid and where are the cos sites derived from. b) The cloning capacity of a cosmid is up to 44 kilo base pairs. State TWO classes of DNA cloning vectors that have higher cloning capacity.

Briefly describe the importance of origin of replication in a cosmid and where are the cos sites derived from. b) The cloning capacity of a cosmid is up to 44 kilo base pairs. State TWO classes of DNA cloning vectors that have higher cloning capacity.

Anatomy & Physiology

1st Edition

ISBN:9781938168130

Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble

Publisher:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble

Chapter3: The Cellular Level Of Organization

Section: Chapter Questions

Problem 3ILQ: Watch this video (http://openstaxcollege.org/l/DNArep) to learn about DNA replication. DNA...

See similar textbooks

Related questions

Q: Based on the picture, why plasmids are used as a vector for DNA Recombination? What other vectors…

A: Ans: Recombinant DNA technology: The given process is the process of recombinant DNA technology in…

Q: A) If one of the primers has a point mutation distinct from the template what is the percentage of…

A: PCR or polymerase chain reaction is a technique which is used in amplification of DNA or produce…

Q: What observation in the Meslson and Stahl experiment to decipher the DNA replication model…

A: There are three models for DNA replication :- A ) Conservative B ) Semi conservative C )…

Q: How many equivalents of ATP are required to replicate a 1000 bp stretch of DNA? B. If the incorrect…

A: Before an answer is given, the process of DNA replication must be understood. First there are two…

Q: Propose a method for isolating a DNA fragment that is adjacent in the genome to a previously…

A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that loop around one…

Q: Draw roughly the comparative electrophoretic mobilities of close circular DNA, open circular DNA and…

A: Since there are multiple question asked in this particular question, I will answer the first one for…

Q: TaqMan probes can specifically measure human DNA concentration, but SYBR Green I cannot-explain.

A: Biotechnology is technology that utilizes biological systems, living organisms or parts of this to…

Q: Reiji and Tuneko Okazaki conducted a now classic experiment in1968 in which they discovered a…

A: The DNA (deoxyribonucleic acid) replication occurs in a semi-discontinuous manner. In this process,…

Q: Draw and explain how the recombinant DNA molecules can be produced in a pUC19 vector using a linear…

A: Introduction Gene cloning is the technique to produce the exact copies of desire gene by utilizing…

Q: Two pathways, homologous recombination and nonhomologous end joining (NHEJ), can repair…

A: Non-homologous end joining (NHEJ) is one important pathway in eukaryotic cells responsible for the…

Q: The 3′-exonuclease activity of E. coli DNA polymerase I was found to show no discrimination between…

A: Introduction: DNA is the type of nucleic acid that is the genetic material of the living body,…

Q: Considering prokaryotes, what is the enzyme that helps hold DNA polymerase III in place when…

A: In prokaryotes, replication is a process by which it makes identical copies of DNA. The process…

Q: Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the…

A: Cell lysis is frequently the very first step in most purification techniques for nucleic acids,…

Q: Describe the process of cloning a DNA fragment into the EcoR1and AluI sites of the vector pUC18. How…

A: DNA cloning is characterized as a cycle of creating different duplicates of a specific DNA. This…

Q: If the cloning was successful and the plasmid contained the elements needed for blue-white…

A: The screening of transformants after the cloning is a critical step as it allows the identification…

Q: Explain the THREE (3) important regions of this plasmid that enable it to work efficiently as a…

A: Plasmid is a self replicating generally circular DNA molecule that can be used to carry a foreign…

Q: What are the essential elements of a plasmid cloning vector? Discuss the importance of each element…

A: Essential elements of plasmid cloning vector Presence of Origin of replication for autonomous…

Q: What characteristics of plasmids makes them good cloning vectors? Discuss.

A: Genetic engineering is the branch of biology which deals with the process of making manipulation in…

Q: What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: the role (presence and absence) of a selectable marker (selection) in cloning a recombinant DNA…

Q: Identify two major functions of DNA pol III in DNAreplication.

A: DNA replication is the natural cycle of producing two indistinguishable replicas of DNA from one…

Q: Explain how DNA polymerase and topoisomerase 2 contribute to replication in E.coli and what is the…

A: It is principally selected to replication-obstructing DNA sores (lesions) to combine the early…

Q: How fast does template DNA spin (expressed in revolutions per second) at an E. coli replication…

A: DNA replication is the process of dividing a double-stranded DNA molecule into two equivalent DNA…

Q: How do we explain the large expected difference in free energy of binding for high fidelity of DNA…

A: The accuracy of DNA replication is most critical to living organisms. The fidelity of replication is…

Q: Discuss the similarities and differences between nucleotide excisionrepair and the mismatch repair…

A: REPAIR PATHWAYS:- Both in Prokaryotes and Eukaryotes, there is a repair enzyme system to deal with…

Q: PLease explain What characteristics of plasmids makes them good cloning vectors? Discuss

A: Plasmids are small extrachromosomal DNA molecules that can reproduce independently of their…

Q: hat is the difference between prokaryotic and eukaryotic DNA polymerase.?

A: A DNA polymerase is an enzyme that helps in the synthesis of DNA molecules by using nitrogenous…

Q: Compare DNA polymerase and RNA polymerase from E. coli in regard to each of the following features:…

A: Introduction: DNA polymerases are the group of enzymes that catalyze the synthesis of DNA strands…

Q: What type of repair would be used to fix a mutation that arises during replication and that escapes…

A: DNA replication is the type of DNA mechanism in which the copies of DNA are made. During the process…

Q: RNA polymeraze binds 100x quicker to the promotor when the promotor is in a 8kb plasmid than to the…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: List and explain the steps of DNA cloning using pUC8.

A: Introduction Molecular cloning is a set of molecular biology experimental methods for assembling…

Q: If you use the pUC18 vector to clone in the MCS region, predict the following: a) Do bacteria that…

A: pUC18 [plasmid of the University of California], is a bacterial plasmid that was derived from pBR…

Q: According to the double-strand break model, does gene conversionnecessarily involve DNA mismatch…

A: Gene Conversion means a modification or conversion of one or two alleles of a gene by the other,…

Q: What is the functional significance of sticky ends in a cloningexperiment?What type of bonding makes…

A: Answer- Sticky ends are generated after restriction endonuclease is applied on a DNA on the…

Q: Explain why a DSB-repair homologous recombination can occur between any two DNA molecules that share…

A: The genetic material that makes up DNA is referred to as the "building block of life." Many…

Q: Assume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: The 3' exonuclease activity of E. coli DNA polymerase I was found to show no discrimination between…

A: DNA polymerase enzyme is involved in the synthesis of DNA. The nucleotides gets added one by one to…

Q: SSBs are: single-stranded DNA binding proteins that prevent re-annealing supercoil stabilizing…

A: DNA replication is the biological process that occurs in every organism, through which they copy…

Q: Describe the process of cloning a DNA fragment into the EcoRI site of the Charon 4 vector. by…

A: Cloning is a procedure to make several identical copies of a DNA (Deoxyribonucleic acid). Charon 4…

Q: How does proofreading differ from later DNA repair mechanisms in terms of timing and the proteins…

A: Proofreading done by DNA polymerase.DNA polymerase detect error at the time of replication and also…

Q: Describe at least three proof-reading mechanisms to achieve High Fidelity of DNA replication.

A: DNA proof-reading is a process of error correction in DNA. It is also called as the DNA repair…

Q: a. What is the purpose of molecular cloning?b. What purpose do selectable markers serve in…

A: Plasmids are small, circular extrachromosomal DNA present in a cell. These extrachromosomal…

Q: What is a plasmid? What are the minimum 3 components that a recombinant plasmid used for genetic…

A: Biotechnology is innovation that uses natural frameworks, living beings or parts of this to create…

Q: What is an STS? How are STSs generated experimentally? What are the uses of STSs? Explain how a…

A: BASIC INFORMATION GENE MAPPING This is a technique to identify the position of elements in the…

Q: With the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining…

A: Molecular cloning or cloning is a technique to assemble DNA of two different organisms and directing…

Q: What are the requirements for in vitro synthesis of DNA under thedirection of DNA polymerase I?

A: The formation of deoxyribonucleic acid molecules, either naturally or artificially, is known as DNA…

Question

a) Briefly describe the importance of origin of replication in a cosmid and where are the cos sites derived from.

b) The cloning capacity of a cosmid is up to 44 kilo base pairs. State TWO classes of DNA
cloning vectors that have higher cloning capacity.

Figure 2 illustrates the important elements of a cosmid.
Cos sites
Cosmid
Origin of replication
Figure 2

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.