Explain why a DSB-repair homologous recombination can occur between any two DNA molecules that share homology rather than only between two DNA molecules that carry a specific sequence.
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Explain why a DSB-repair homologous recombination can occur between any two DNA molecules that share homology rather than only between two DNA molecules that carry a specific sequence.
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- With regard to the repair of double-strand breaks, what are the advantages and disadvantages of homologous recombination repair (HRR) versus nonhomologous end joining (NHEJ)?Two pathways, homologous recombination and nonhomologous end joining (NHEJ), can repair double-strandbreaks in DNA. If homologous recombination is an errorfree pathway whereas NHEJ is not always error free, whyis NHEJ used most of the time in eukaryotes?With regard to the repair of double-strand breaks, what are the advantages and disadvantages of homologous recombination repair versus nonhomologous end joining?
- Homologous recombination in E. coli forms heteroduplex regions of DNA containing mismatched bases. Why are these mismatches not eliminated bythe mismatch repair system?Which of these two changes is more difficult for DNA repair enzymes to fix correctly? Explain why.Mismatch repair in E. coli distinguishes between old and new strands of DNA on the basis of a. differences in the base composition of the two strands. b. modification of histone proteins. c. base analogs on the new strand. d. methyl groups on the old strand.
- Eukaryotic cells can repair DNA through a process called homologous recombination. What type of DNA damage can be fixed through homologous recombination?What is the advantage of homology-directed repair (HDR) ?Explain why the absorption of UV light by double-stranded DNA increases (the hyperchromic effect) when the DNA is denatured.
- Which DNA repair systems you think might be capable of repairing a situation where T is in one strand and G is in the complementary strand? Explain dramatically.a. Why doesn't Dpnl digest the mutagenized DNA? b.What is the approximate size, in base pairs, of the pQE.1-CRYGD plasmid?Define the following terms:a. nonreplicative transpositionb. replicative transpositionc. composite transposond. retrotransposone. insertional element