Calculate the unknown concentration of the PROTEIN A with an absorbance value of A188 given the standard curve indicated in the table below. Write your final answer (NUMBER ONLY) in two decimal places rounded off.
Q: Answer the numbers 1 & 2 1. Positive with Molisch Test and Benedict Test but negative with both…
A: Carbohydrates or carbs are maconutrient consisting of Carbon, hydrogen and oxygen atoms. In nature…
Q: Which of the following organs does glycolysis occur in an anaerobic environment?* a.Liver b.Brain…
A: The glycolytic pathway metabolizes the glucose molecules to produce energy. The glycolytic pathway…
Q: When one acetyl CoA is processed through the citric acid cycle, how many times does each of the…
A: In aerobic condition, pyruvate in the presence of pyruvate dehydrogenase complex produces Acetyl…
Q: Choose the INCORRECT statement: Group of answer choices All statements are incorrect. Each ribosome…
A: The ribosome is a translation machine that with the help of translation factors and amino acyl tRNAs…
Q: Define the pedigree symbol(s) associated with each of the following individuals. Please be as…
A: Pedigree: Pedigree is a family tree that is used to show the mode of inheritance of genetic traits/…
Q: Which of the following are examples of lipids? A. DNA, RNA, ATP B. triglycerides, waxes, steroids,…
A: Biological macromolecules are the massive compounds required for life that are constructed from…
Q: 5' UGG CAA UCC UAC GAU 3' - 1. Here is the MRNA sequence from a section of a gene (it is the middle…
A: Note : Hi ! Thank you for the question. We are authorized to answer three subparts at a time. Since…
Q: 4. Fill in the table below Pathway Glycolysis Krebs Cycle Electron Transport Chain Number of ATP…
A: Glucose is a carbohydrate that is catabolized by glycolysis into two molecules of pyruvate. Pyruvate…
Q: If the pH of a voledronic acid solution is 5.8, and the voledronate concentration is 9 mM, what is…
A: Henderson Hasselbalch equation can be used to determine the concentration of a weak acid in an…
Q: In the Baltimore Classification of Viruses, viruses with a genetic material in the form of RNA are…
A: The virologist and Nobel laureate David Baltimore established the Baltimore method of virus…
Q: Draw a schematic diagram on glycogenolysis by showing the structural formula of its substrate. Show…
A: Glycogen is mainly the "storage" form of carbohydrates present in mammals. Majorly in humans, the…
Q: Using a generalized terminology (i.e. neither CIII nor CIV specific), explain how complex III and IV…
A: The transfer of electrons from one complex to another results in the release of protons to the…
Q: in the processing 1 molecule of pyruvate what will the atp yield is complex II of the electron…
A: Generally, 14 molecules of ATP are produced in processing one molecule of pyruvate. In the first…
Q: Explain what immunoaffinity extraction consists of when applied to obtaining steroids
A: Immunoaffinity chromatography (IAC) combines the use of Liquid chromatography LC with the specific…
Q: TRUE or FALSE: "The initiation codon for both prokaryotes and eukaryotes is AUG coding for the same…
A: Introduction: The genetic code consists of 64 triplets o nucleotides. These triplets are referred to…
Q: A central tenet in mitochondrial bioenergetics is that exergonic electron transfer drives the…
A: ETC is series of steps that carry the transport of electron from electron donors NADH to electron…
Q: Which of the following indicates buffering in the titration given in the image below? 12 1000000 11…
A: A buffer is a aqueous solution which is used to resist the pH change upon addition of acid or base.…
Q: Cytoplasm should have a higher concentration of _____ to help glycolysis going. NAD+ ATP…
A: Nicotinamide adenine dinucleotide is a coenzyme plays a central role in metabolism. It is found all…
Q: Identify what type the volume in the ITEM 8 in the diagram. Choose the letter of the correct answer…
A: Micropipeptes are tools used to dispense volumes of liquid in microliters.
Q: 7. How does enzyme inhibition occur? Give and illustrate the reaction between enzyme and substrate…
A: Enzyme inhibition occurs when there is inhibition of the conversion of the substrate to product due…
Q: Macromolecule Proteins Amino +H H H Nucleic Acids phe Carboxyl 0 Monomer(s)/ Subunit(s) and Name of…
A: There are 4 types of Biomacromolecules. They are; Proteins Nucleic Acids Carbohydrates and Lipids…
Q: how the function of two or three different lipids is connected to their structure. be meaningful; ●…
A: Introduction: Macromolecules are large molecules within the cells. It is made from thousands of…
Q: which of the following are possible sources of glyceraldehyde 3-phosphate a. more than 1 correct…
A: Glycolysis is defined as a series of enzymatic reactions that convert one molecule of glucose, a…
Q: The definitive host of human malaria parasites are certain species of Anopheles mosquitoes. True…
A: Malaria is caused by a parasite known as Plasmodium, which is normally spread through infected…
Q: QUESTION : Which of the following best describes the transition state of a catalyzed reaction? A.…
A: Enzymes are mostly proteins that hasten the chemical reactions in our bodies.
Q: what will happen (increase, decrease or no effect) to the activity of enzyme or rate of the…
A: Aleration of The activity of enzyme or rate of the metabolic pathway for given conditions are :
Q: in a clever experiment performed in 1962, a cysteine already attached to its tRNA was chemically…
A: The tiny RNA molecule transfer RNA (abbreviated tRNA) is essential for protein synthesis. Transfer…
Q: Carbonic anhydrase has an acidic zinc canter compared to carboxypeptidase. Mention the statement is…
A: Carboxypeptidase A is a hydrolase that hydrolyzes the peptide bond at a polypeptide or protein's…
Q: Multiple choice 1. Which of the following biomolecules is a lipid? A. cellulose B. protein C.…
A: lipids:- Biological lipids are chemically diverse group of organic compounds, & are insoluble…
Q: In receptor mediated endocytosis, the receptors (such as the low-density lipoprotein, LDL, receptor)…
A: The plasma membrane regulates the entry and exit of the molecules across the membrane. The small…
Q: What is the clinical AST?
A: AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate in liver.
Q: Discuss the glycogen synthesis. Give the three enzymes required for this process and their…
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: How to use CRISPR cas 9 system to solve a problem or disease in molecular biology
A: CRISPER cas9 is the gene-editing tool that edits the parts of the genome by removing, adding, or…
Q: What are the different major macromolecules? How are they interrelated to each other? Is there a…
A: Introduction: Macromolecules are larger molecules that are made from smaller ones. Many are polymers…
Q: Which pathways produce NADPH
A: NADPH means nicotinamide adenine dinucleotide phosphate. NADPH is also known as reducing equivalent.…
Q: Comparison between palmytic acid and oleic acid
A: Fatty acids are referred to as the building blocks of the fats that are present in our bodies.…
Q: 1. make your own version of Lipid Metabolism Cycle with diagram. 2. explain why these cycles are…
A: Glycolysis, TCA cycle, and ETC all are interconnected processes. Metabolism includes biosynthesis/…
Q: A high serum total protein together with low albumin levels is usually seen in which of the…
A: The measurement of total serum protein and albumin are of diagnostic importance in the treatment of…
Q: Find the connection among the words below and choose the letter of the word which is different.
A: Electrophoresis is a laboratory technique for sorting DNA, RNA, and protein molecules according to…
Q: Explain briefly but concisely 2. How would riboflavin deficiency affect the functioning of the…
A: Riboflavin is also called vitamin B2. It is a water-soluble vitamin. Through its coenzymes, it…
Q: 1. Major controls of de novo AMP synthesis include: 1. allosteric inhibition by GMP. 2. allosteric…
A: De novo synthesis is the process of creating complex molecules from simple molecules such as sugars…
Q: 4. Use the cartoon protein below to answer the following questions. Assume the rounder subunits are…
A: Proteins are polymers of amino acids linked by peptide/amide bond (Covalent). Primary Structure of…
Q: plant foods often contain oxalates and phytates. what effects do these have on minerals that are…
A: Phytates and oxalates are present in plant foods. Phytates are other wise called anti nutrients…
Q: How can enzyme inhibition be used in the treatment of certain diseases? Cite examples
A: Introduction: Inhibitors are substances that bind to an enzyme and inhibit its activity. It will…
Q: The shape for curve of oxygen binding to Myoglobin is ___________ and the shape for curve of oxygen…
A: Because of their distinct structure, myoglobin and hemoglobin have slightly different…
Q: The G-proteins associated with GPCRS are made up of three subunits. In order to become active they…
A: The G protein coupled receptors are a classes of cell surface receptors. They are associated with G…
Q: A Carhon present eny in eecleic acide BOvygen C Nitrogen D. Phosphorus 19 Which anture pre proteins…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Which of the following functions describes the enzyme Peptidase? A. remove phosphate from a…
A: Peptidase are enzymes that comes under the category of hydrolase that is class 3 of enzymes i.e.,…
Q: What happens when a stop codon is reached by a ribosome? A termination tRNAter binds to the codon…
A: The protein synthesis process is known as translation. The protein synthesis occurs in the cytoplasm…
Q: 10. Draw the condensed structural formula for the a-keto-acid produced from each of the following in…
A: Transamination is a chemical reaction that transfers an amino group to a keto-acid leading to the…
44
Trending now
This is a popular solution!
Step by step
Solved in 3 steps with 1 images
- If a protein lysate obtained is 1003 ug/ml What would be the total concentration of each lysate that is loaded into their well if you load just 25 ul of each?Using the equation for the best-fit straight line through your data, the average absorbance of your unknown samples U1 and U2, and any dilution factors (DON’T LEAVE OUT THE DILUTION FACTOR), calculate the concentration of protein in the original unknown protein sample. y = 1.6849x + 0.0414R² = 0.9904q44 please calculate the unknown concentration of the protein A wih an absorbance value of A188 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762
- Total protein: Do you think that refractive index could be used as a regular method for serum protein determinations? If yes, what limitations does it have. If not, why not? What are the major interferences in the biuret method? Refractometry method?In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the absorbance of the serum sample is determined as 0.28, what is the amount of cholesterol in the sample, in mg/L?After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.
- Using the data from the standard curve done previously, you record the absorbance of your extracted milk protein sample at 0.499 at 540nm. What is the mass of the protein in mg assuming you collected 10mL worth of the protein? Your standard curve should have a line of best fit with the equation y=0.0001x+0.0038.Calculate protein concentration in unknown samples 1, 2, 3: Absorbance of Unknown 1 = 0.541 Absorbance of Unknown 2 = 0.85 Absorbance of Unknown 3 = 1.02 Standard Curve: Y = 0.0073x1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.
- The following protein quantification utilizes Cu ions except fora. Lowryb. Biuretc.BradfordThe protein concentration of a known standard is 100mg/mL If you prepared a serial dilution, mixing 10μL of protein with 40μL of water what would be concentrations of the first 3 dilutions?KCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?