calculate the values of the rate constant k-1, and the value of the dissociation constant of the ES-Complex, Ks with appropriate units 2) calculate the total amount of enzyme used in the experiment and the catalytic efficiency ratio

Appl Of Ms Excel In Analytical Chemistry
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ISBN:9781285686691
Author:Crouch
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Chapter13: Kinetic Methods
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1) calculate the values of the rate constant k-1, and the value of the dissociation constant of the ES-Complex, Ks with appropriate units 2) calculate the total amount of enzyme used in the experiment and the catalytic efficiency ratio
3. An experiment with Carbonic anhydrase (CA), which catalyses the interconversion between
carbon dioxide and carbonic acid results in the following data:
k₁= 5 x 10² M¹¹ s¹
k₂ = 0.5 x 105 s-¹
KM = 0.01M (Briggs-Haldane model)
Vmax = 45 mM.s¹
K₁
k₂
E+S ES E + P
K-₁
Transcribed Image Text:3. An experiment with Carbonic anhydrase (CA), which catalyses the interconversion between carbon dioxide and carbonic acid results in the following data: k₁= 5 x 10² M¹¹ s¹ k₂ = 0.5 x 105 s-¹ KM = 0.01M (Briggs-Haldane model) Vmax = 45 mM.s¹ K₁ k₂ E+S ES E + P K-₁
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