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Can you be sure that after 6.5 hours all the cells are viable? What would you do to determine the viability at these times
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- A culture with approximately 3x104 cells/mL were incubated. After 3 hours, the number of cells had increased to 6x105 a) How many generations have occurred?You are doing a viable cell count for a culture of E. coli you are growing in a flask. The volume of media in the flask is 1L. You took an aliquot directly from the media and diluted it using trypan blue. Here are the numbers that you get: squares counted: 4 total cells counted: 988 total viable cells counted: 960 THESE ARE THE QUESTIONS: What is your cell viability? What is the total number of viable cells in your flask? Show all work. Given: Cell Viability = Viable Cells/Total Cells Cells/mL = Total Viable Cells/Coners Counted x 10,000 x Dilution factorWhy is the plate count method a determination of the number of viable cells?
- You are incubating cells for 3 hours. The initial number of cells present in the sample were 102. After the incubation period, you find that the culture now has 104 cells. What is the generation time in minutes?A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109. a) How long was the generation time in minutes?b) How many generations have occurred?If a broth tube is inoculated with 100 cells which immediately enter exponential growth, how many cells will there be after 8 hours if the doubling time is 30 minutes?
- A broth tube is inoculated at 9 AM. At 12 PM there are 6,400 cells. At 2 PM there are 400,000 cells. How many cells were put in the tube?Which cells are or have been made totipotent? Select all that apply A) a zygote B) sperm cells C) plant cells in the right experimental medium D) human skins cells in the right experimental mediumYou are growing a culture of S. aureus. You know that 9 generations have occurred after 6 hours. What is the generation time in minutes? if i started a culture with 1000 cells, after 8 generations, how many cells will i have?
- You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?If a flask is inoculate with 10 E. coli cells. After 6 hours you now have 200,000 cells. determine both n (number of generations) and g (generation time) in minutes? A culture of S. epidermidis is growing. It divides every 20 min. After 4 hours, how many generations occurred?Suppose that you were tasked with 4 cultures of a specific Bacteria Species in Luria Broth medium: (1) culture A – cells are in lag phase; (2) culture B – cell in log phase; (3) culture C – cells in stationary phase; and culture D – cells in decline phase. Imagine that you observe the growth rates of each culture in a fresh sterilized LB medium. Now, plot the outcome growth curves of cultures A, B, C and D in a single graph.