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A broth tube is inoculated at 9 AM. At 12 PM there are 6,400 cells. At 2 PM there are 400,000 cells. How many cells were put in the tube?
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- Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth culture, removed the cap, and flamed the mouth of the tube. He inserted an inoculating loop to obtain a bacterial sample. Then, he flamed the mouth of the tube and replaced the cap. Noah opened the lid of a labeled agar plate diagonally and used the loop to streak the surface of the agar. After closing the lid, he flamed the loop in an incinerator and put it back in its container. The plate was incubated upside down for 24–48 hours. What did Noah do wrong in this transfer? a. He did not use the transfer tool correctly. b. He did not handle the culture tube correctly. c. He did not handle the agar plate correctly. d. He used the wrong tool in transfer.Using saline tubes containing 9 ml of saline (for dilutions), draw a diagram that would yield between 30 and 300 colonies from an original broth culture that contains 5 * 10^8 cells/ml. Indicate volumes used and dilutions made for each tube and plate.You have aseptically inoculated an unknown organism on a blood agar plate. You return 48 hours later and the agar is no longer red and opaque but now is yellow and transparent where the bacteria has grown. You conclude your organism exhibits... A. Alpha hemolysis B. Gamma hemolysis C. Beta hemolysis D. No lysis
- A broth containing 1 million bacterial cells is treated with an antimicrobial agent that kills 90% of the cells in 1 minute. What is the minimum amount of time it will take before all the cells in the broth are dead? 3 minutes 5 minutes 7 minutes 10 minutesWhich test tube is the control group? Test tube 1: 5 Hydrogen peroxide only Test tube 2: 5 Hydrogen peroxide and ground potato Test tube 3: 5 Hydrogen peroxide and diced potato Test tube 4: 5 Hydrogen peroxide And boiled potatoA broth culture is initially inoculated with a single bacterial cell. For the first hour the bacteria has a generation time of 30 minutes, for the second hour the generation time has been reduced to 15 minutes, and for the third hour the generation time has been reduced to 10 minutes. At the end of the third hour how many bacteria are present in the broth culture?
- Surgical instruments are cleaned by heating them. Alcohol is used as disinfectant in cleansing the skin before inoculation. Why are these methods successful in killing harmful germs?What color would you expect for a gram positive microorganism for each of his incorrect attempts? mistake: forgot the alcohol wash step.As you can see on the blood agar plates, some microbes can lyse red blood cells releasing nutrients into the surrounding area. What might the advantage be for a bacteria to be able to do this? Do you think this could have an effect on the bacteria’s virulence? Explain.
- You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the nutrient agar plates after incubation and gave you 24 colonies. What is the CFU/ml? What is a CFU?You perform a nitrate test. After adding solutions A and B, the broth remains colorless. Why should you not interpret this as a negative test? What do you do next, and how do you interpret the results?why would selecting a colony from a blood agar plate to test with enterotube be bad