If a broth tube is inoculated with 100 cells which immediately enter exponential growth, how many cells will there be after 8 hours if the doubling time is 30 minutes
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If a broth tube is inoculated with 100 cells which immediately enter exponential growth, how many cells will there be after 8 hours if the doubling time is 30 minutes?
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- Why would cells that are vigorously growing when inoculated into fresh culture medium have a shorter lag phase than those that have been stored in a refrigerator?A bacterial culture contains 500 cells/mL in the exponential growth phase at 8 AM. If you onsider a generation time of 20minutes, what will be the total number of cells/mL after three hours?Calculate the mean generation time of a bacterium that increased its number from 1x10^3 cells/ml to 5x10^8 cells/ml in 10 hrs.
- If the generation time is 90 min and the initial population contains 103 cells, how many bacteria will there be after 8 hours of exponential growth?Calculate the growth rate and generation time of a culture that increases in the exponential phase from 5x102 to1x108 cells in 12 hoursAssume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xo
- The generation time of microbacterium cells present in activated sludge is 30 minutes. If these bacteria are allowed to grow for 15 hours, how many generation would have taken placeCalculate the growth rate and generation time of a culture that increases in the exponential phase from 5 x 102 to 1 x 108 cells in 12 hours.Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.
- A 23 year old woman has 10 E coli in her bladder. The organism has generation time of 20 minutes. After a lag of 20 minutes, the E. coli entered the log phase of growth. After 3 hours, what is the total number of cells?If the original number of cells in a sample was 12,000, how many bacteria will be present in 12 hours when the generation time is 15 minutes?A slant is inoculated with 100 E.coli cells. The slant is left at room temperature where the generation time is 1 hour. How many E. coli are on the slant in 6 hours? If the slant was initially put in the 37C incubator, with a 20 minute generation time, how many more cells would have grown in the same time period?