14.25 You are studying a circular plasmid DNA molecule ofsize 10.5 kilobase pairs (kb). When you digest this plas-mid with restriction endonucleases BamHI, EcoRI, andHindIII, singly and in all possible combinations, youobtain linear restriction fragments of the following sizes:EnzymesBamHIEcoRIHindIIIBamHI + EcoRIBamHI + HindIIIEcoRI + HindIIIBamHI + EcoRI + HindIIIFragment Sizes (in kb)7.3, 3.210.55.1, 3.4, 2.06.7, 3.2, 0.64.6, 2.7, 2.0, 0.7, 0.54.0, 3.4, 2.0, 1.14.0, 2.7, 2.0, 0.7, 0.6, 0.5Draw a restriction map for the plasmid that fits yourdata.

The triple digestion data can be compared with double digestion and single digestion data and…

Enzymes BamHI EcoRI HindIII BamHI + EcoRI BamHI + HindIII EcoRI + HindIII BamHI + EcoRI + HindIII Fragment Sizes (in kb) 7.3, 3.2 10.5 5.1, 3.4, 2.0 6.7, 3.2, 0.6 4.6, 2.7, 2.0, 0.7, 0.5 4.0, 3.4, 2.0, 1.1 4.0, 2.7, 2.0, 0.7, 0.6, 0.5 Draw a restriction map for the plasmid that fits your data.

Enzymes BamHI EcoRI HindIII BamHI + EcoRI BamHI + HindIII EcoRI + HindIII BamHI + EcoRI + HindIII Fragment Sizes (in kb) 7.3, 3.2 10.5 5.1, 3.4, 2.0 6.7, 3.2, 0.6 4.6, 2.7, 2.0, 0.7, 0.5 4.0, 3.4, 2.0, 1.1 4.0, 2.7, 2.0, 0.7, 0.6, 0.5 Draw a restriction map for the plasmid that fits your data.

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter9: Molecular Biology

Section: Chapter Questions

Problem 1ACQ: Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired...

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Question

14.25 You are studying a circular plasmid DNA molecule of
size 10.5 kilobase pairs (kb). When you digest this plas-
mid with restriction endonucleases BamHI, EcoRI, and
HindIII, singly and in all possible combinations, you
obtain linear restriction fragments of the following sizes:
Enzymes
BamHI
EcoRI
HindIII
BamHI + EcoRI
BamHI + HindIII
EcoRI + HindIII
BamHI + EcoRI + HindIII
Fragment Sizes (in kb)
7.3, 3.2
10.5
5.1, 3.4, 2.0
6.7, 3.2, 0.6
4.6, 2.7, 2.0, 0.7, 0.5
4.0, 3.4, 2.0, 1.1
4.0, 2.7, 2.0, 0.7, 0.6, 0.5
Draw a restriction map for the plasmid that fits your
data.

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