67. To make an insertion using CRISPR Cas9, one must include a DNA with flanking sequences similar to the desired target sequence to take advantage of the endogenous DNA repair mechanism known as ______________________.
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67. To make an insertion using CRISPR Cas9, one must include a DNA with flanking sequences similar to the desired target sequence to take advantage of the endogenous DNA repair mechanism known as ______________________.
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- 63. DNA sequence specificity of the CRISPR Cas9 complex is determined by: Group of answer choices None of these answers. guide RNA guide DNA primers mostly protein mediatedProvide a brief summary of the Sanger sequencing method.Quinolone antibiotics treat bacterial infections by blocking the activity of topoisomerase. Why does this treatment work? Explain what occurs at the molecular level.
- Below is a sequence of DNA. 5'-ttaccgataattctctctcccctcttccatgattctgattaaagaaggcgagaacgaaactatttgttaatacc-3' How many "reading frames" can be identified for this sequence? How many "open reading frames" can be identified for this sequence? What is the frame of the longest ORF?45. Which of the following steps are required for cDNA production from mRNA? MARK ALL THAT APPLY. Group of answer choices Protein synthesis Sodium hydroxide degradation of mRNA PCR using poly-dT and poly-dC primers RNA synthesis IntronsThese are written as either accurate or contain errors. Rewrite each one with an error as an accurate statement. Please have an explanation. Thank you! An insertion mutation is not as severe as a transversion because the transversion changes the reading frame within the RNA. SINES, LINES and SNPs are three common types of repetitive sequences.
- 8a. Given the following mutated sequence (with respect to the normal sequence), what TYPE of mutation occurred: AAACGTTAC 8b. Where did the mutation take place?why DNA polymerase cannot remove all the RNA primers from Okazaki fragments to form a lagging strand. Explain.The following are DNA fragments containing a small gene. The top strand is the coding strand. Transcribe all groups and translate. FIND THE POSSIBLE MUTATIONS Group B - MUTATION 5’-GGCAATGGGTTTGTGAAATTCTAAAAGTTTTTAATTC-3’ 3’-CCGTTACCCAAACACTTTAAGATTTTCAAAAATTAAG-5’ Group C- 5’-GGCAATGGGTTTGTGCAATTCTAAGAGTTTTTAATTC-3’ 3’-CCGTTACCCAAACACGTTAAGATTCTCAAAAATTAAG-5’
- 51. In 1973, Cohen and Boyer created the first chimera by successfully incorporating the frog rRNA into bacteria. The restriction endonuclease EcoRI was used to cut the frog DNA that contained the rRNA gene and the vector plasmid pSC101. The DNA fragments and the vector plasmid pSC101 were combined and transferred to E. coli bacteria.The frog DNA fragment and pSC101 were able to combine when Select one: a. a pyrimidine base in the sticky end of the DNA fragment came in contact with a pyrimidine base in pSC101 b. a nucleotide in the sticky end of the DNA fragment came in contact with a complementary base in pSC101 c. a nucleotide in the sticky end of the DNA fragment came in contact with the same base in pSC101 d. a purine base in the sticky end of the DNA fragment came in contact with a purine base in pSC101Given Sequence: 3’ – TACGGACTGATAGGCCCGCGCATC-5’ PLEASE PROVIDE THE RANSLATION OF THE FOLLOWING: a.Complementary Strand: b. Direct Transcript: c. Transcript for Translation: d.Translated Amino Acid Sequence:6a. Given the following mutated sequence (with respect to the normal sequence), what TYPE of mutation occurred: AAGCTTAC