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a) What are the methods used for cholesterol determination? Explain the basis of the method you used in your experiment by writing.
b) In the experiment conducted for the determination of cholesterol, the function of the calibration curve obtained using standard cholesterol solutions of 25-400mg/dl was determined as y= 0.0009x.Since the absorbance of the serum sample was determined as 0.28, how many mg/L is the amount of cholesterol in the sample
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- In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the absorbance of the serum sample is determined as 0.28, what is the amount of cholesterol in the sample, in mg/L?What level of RF in serum is clinically significant? Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the qualitative test? Describe how the RF concentration is computed?If a pharmacist added each of the following to 22-g packages of 2% mupirocin ointment, what would be the percentage strengths of the resulting ointments: (a) 0.25 g mupirocin powder and (b) 0.25 g of nonmedicated ointment base? (answer to two decimal places). answers: (a) 3.10% w/w (b) 1.98% w/w
- In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the amount of cholesterol in the serum sample is determined as 3.11 g/L, what is the absorbance value of the sample? A. 0,280 B. 0.028 C. 0.208 D. 0.82. How much patient serum is needed to make a 1 mL 1:25 dilution? b. If a test procedure requires that the patient serum be diluted with 7 parts of distilled water, how much diluent should be used if the required serum volume is 250 µL? What is the final volume and dilution factor of the solution? c. How much NaCl in grams and distilled water is needed to make 10 mL of 0.9% NaCl solution? d. What is the final dilution factor of a solution if 500 µL serum is diluted with 9.5 mL of distilled water?In the ESTIMATION OF TOTAL SERUM CHOLESTEROL using ZAK AND HENLY’S METHOD, explain why should the serum stay clear? What are the implications of using a turbid serum sample?
- What results are expected in the Molisch's test? What is the test for?Total protein: Do you think that refractive index could be used as a regular method for serum protein determinations? If yes, what limitations does it have. If not, why not? What are the major interferences in the biuret method? Refractometry method?If 400 mL of a 20% w/v solution were diluted to 2 L, what would be the finalpercentage strength?
- The physician has ordered 500,00 units of IV Pfizerpen every 4 hours. How much diluent will you add for a concentration of 500,000 units/mL? How many milliliters will you administer?The drug dosage for the patient is calculated using a BSA of 1.9m2. If the order is 15mg per m2, how many milligrams is the desired dose?How much serum is needed to create 2μL of a 1:20 dilution?