In the ESTIMATION OF TOTAL SERUM CHOLESTEROL using ZAK AND HENLY’S METHOD, explain why should the serum stay clear? What are the implications of using a turbid serum sample?
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In the ESTIMATION OF TOTAL SERUM CHOLESTEROL using ZAK AND HENLY’S METHOD, explain why should the serum stay clear? What are the implications of using a turbid serum sample?
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- In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the absorbance of the serum sample is determined as 0.28, what is the amount of cholesterol in the sample, in mg/L?a) What are the methods used for cholesterol determination? Explain the basis of the method you used in your experiment by writing.b) In the experiment conducted for the determination of cholesterol, the function of the calibration curve obtained using standard cholesterol solutions of 25-400mg/dl was determined as y= 0.0009x.Since the absorbance of the serum sample was determined as 0.28, how many mg/L is the amount of cholesterol in the sampleTotal protein: Do you think that refractive index could be used as a regular method for serum protein determinations? If yes, what limitations does it have. If not, why not? What are the major interferences in the biuret method? Refractometry method?
- How to estimate serum sialic acid can you provide method protocol for sialic acid estimation?What is a folin-ciocalteau reagent and how can it be used to determine the concentration of uric acid in a blood serumExplain why glycated haemoglobin (HbA1C) is a suitable index for long term hyperglycaemia.
- A cholesterol screening has reported Toms blood serum contains 50mg/dal HDL and 180 mg/dal LDL and a total cholesterol of 255. What is the LDL/HDL ratio? Is this value considered in the healthy range?What results are expected in the Molisch's test? What is the test for?After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.
- What is the typical composition of Harris Hematoxylin Solution? Which cellular organelle was more visible after immersing the intestinal cells in hematoxylin?What determines plasma levels of 1,25(OH)2D3?Plasma or serum LDL-Cholesterol (LDL-C) can be calculated by determining concentrations of total plasma (serum cholesterol (T-C), HDL-Cholesterol (HDL-C) and total plasma (serum) triglycerides(TG) using Friedewald equation: LDL-C = (T-C) - (HDL-C) - TG/5. What is the scientific basis for this equation? What are the limitations of this equation?
