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Choose a protein and describe how the protein shows different detergents take proteins selectively out of membrane

Choose a protein and describe how the protein shows different detergents take proteins selectively out of membrane

Curren'S Math For Meds: Dosages & Sol
Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 33SST
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Choose a protein and describe how the protein shows different detergents take proteins selectively out of membrane
top bottom top bottom top bottom VIP17/MAL PLAP caveolin-1 stomatin Yes TfR gp114 calnexin rab-5 Lubrol WX Brij 96 Triton association of marker proteins from MDCK cells. MDCK-PLAP cell homogenate was extracted with 0.5% Lubrol WX, 0.5% Brij 96 n linear sucrose gradients. The distribution of marker preins was analyzed by immunoblotting. The light fractions from the top c t, the heavy bottom fractions on the right. ally solubilized gp114, most of TfR, and 50% of Yes ter), whereas Triton solubilized rab-5, calnexin, gp114, nost of Yes (Fig. 2 Right). Hence, DRM association of ker proteins confirms the graded selectivity of the observed by analysis of the plasma membrane DRM ntent. ults with Triton agreed with earlier observations (6, 11, Regarding Lubrol WX, calnexin had previously been phospholipid and cholesterol/glycerophospholipid calculated. The fold changes of these ratios relative t are depicted in Fig. 4. No significant changes occ higher detergent concentrations were used or wi where prepared by pelleting from isolated membrane flotation from cell homogenate (not shown). Compared with TCMS, DRMS from MDCK cell with Triton and CHAPS showed clear enrichment c
Transcribed Image Text:top bottom top bottom top bottom VIP17/MAL PLAP caveolin-1 stomatin Yes TfR gp114 calnexin rab-5 Lubrol WX Brij 96 Triton association of marker proteins from MDCK cells. MDCK-PLAP cell homogenate was extracted with 0.5% Lubrol WX, 0.5% Brij 96 n linear sucrose gradients. The distribution of marker preins was analyzed by immunoblotting. The light fractions from the top c t, the heavy bottom fractions on the right. ally solubilized gp114, most of TfR, and 50% of Yes ter), whereas Triton solubilized rab-5, calnexin, gp114, nost of Yes (Fig. 2 Right). Hence, DRM association of ker proteins confirms the graded selectivity of the observed by analysis of the plasma membrane DRM ntent. ults with Triton agreed with earlier observations (6, 11, Regarding Lubrol WX, calnexin had previously been phospholipid and cholesterol/glycerophospholipid calculated. The fold changes of these ratios relative t are depicted in Fig. 4. No significant changes occ higher detergent concentrations were used or wi where prepared by pelleting from isolated membrane flotation from cell homogenate (not shown). Compared with TCMS, DRMS from MDCK cell with Triton and CHAPS showed clear enrichment c
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