1. To produce a specialized-transducing mycobacteriophage construct, a cosmid bearing the allelic exchange substrate is electroporated in Mycobacterium smegmatis, followed by an incubation at 30°C. True/ False 2. A recombineering system involves the over-expression of recombinase proteins, which allows the use of a short DNA fragment as allelic exchange substrate, but can also be combined with the use of a mycobacteriophage construct. True/ False

1. To produce a specialized-transducing mycobacteriophage construct, a cosmid bearing the allelic exchange substrate is electroporated in Mycobacterium smegmatis, followed by an incubation at 30°C. True/ False 2. A recombineering system involves the over-expression of recombinase proteins, which allows the use of a short DNA fragment as allelic exchange substrate, but can also be combined with the use of a mycobacteriophage construct. True/ False

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 5TYK

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(1) Why the ratio of PE/GFP fluorescence is measured in the FACS experiment, instead of measuring only total PE fluorescence? (2) Explain the effect that each mutation causes in the function of MC4R, and how they can be linked to disease.
1. Hemagglutination and hemagglutination inhibition assays can be used for the following purposes, EXCEPT: Demonstration of presence of hemagglutinin-expressing virus Demonstration of presence of neuraminidase-expressing virus Finding out the serotype of virus Finding out serotype of virus antibodies Demonstration of infectivity of virus 2. Virus may acquire resistance to antiviral chemotherapy through the following mechanisms, EXCEPT Mutation Recombination Transduction Transformation Reassortment
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