coli, mak you believe will grow faster (37° C or 30° C temperature) and why. Results• Time Absorbance 30 °C Absorbance 37 °C Generation time Generation time t=0 min at 30 °C at 37 °C 0.05 0.05 t= 20 min 0.06 0.07 t= 40 min %3D 0.1 0.16 0.28 0.21 0.44 t = 60 min t= 80 min %3D 0.95 Complete the graph. Make sure this figure has a description and all other labels are included. Once you have completed graphing the points, draw a best-fit line for each of your data sets by eye Questions: 1. Which data point can be the first to be considered in log phase for each line? (It will be the first point that seems to be described by your best-fit line.) For the 30° C culture the -minute point is the first one likely to be in log phase. For the 37° C culture the -minute point is the first one likely to be in log phase. Incubation Temperatures - Growth Bio 40- Fall 2019 Page 6

coli, mak you believe will grow faster (37° C or 30° C temperature) and why. Results• Time Absorbance 30 °C Absorbance 37 °C Generation time Generation time t=0 min at 30 °C at 37 °C 0.05 0.05 t= 20 min 0.06 0.07 t= 40 min %3D 0.1 0.16 0.28 0.21 0.44 t = 60 min t= 80 min %3D 0.95 Complete the graph. Make sure this figure has a description and all other labels are included. Once you have completed graphing the points, draw a best-fit line for each of your data sets by eye Questions: 1. Which data point can be the first to be considered in log phase for each line? (It will be the first point that seems to be described by your best-fit line.) For the 30° C culture the -minute point is the first one likely to be in log phase. For the 37° C culture the -minute point is the first one likely to be in log phase. Incubation Temperatures - Growth Bio 40- Fall 2019 Page 6

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Assume tu inoculated 100 facultative anaerobic cells unto nutrient agar and incubated the plate aerobically. You then inoculated 100 cells of the same species unto nutrient agar and incubated the second plate anaerobically. After incubation for 24 hours, you should theoretically have: A) More coloneis on the aerobic plate B) More colories on the anaerobic plate C) The same number of colonies on both paltes D) Fewer colonies on the anaerobic plate E) None of the above
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A 3.0 L batch bioreactor is used to grow E. coli bacteria, which have an initial concentration of 12.5 g cells/L. The growth medium contains two carbon sources, which causes diauxic growth, with each carbon source limiting cell growth. Sufficient supplies of oxygen, ammonium nitrate (NH4NO3) as a nitrogen source and all other macro and micronutrients are available. The bacteria prefer to grow on glucose (C6H12O6, supplied at 80 g/L), so it is consumed first, followed by glycerol (C3H8O3, supplied at 68 g/L). Since the E. coli were already growing on glucose, the lag time associated with glucose consumption is only 10 minutes. The lag time associated with glycerol consumption is 35 minutes. Further information: The chemical formula for E. coli in this bioreactor is CH1.666N0.20O0.57. The specific growth rate on glucose is 0.41 h-1 , and on glycerol is 0.32 h-1 . The yield of cells on glucose is 0.48 g cells/g glucose, and yield on glycerol is 0.39 g cells/g glycerol. You may assume that…
A 3.0 L batch bioreactor is used to grow E. coli bacteria, which have an initial concentration of 12.5 g cells/L. The growth medium contains two carbon sources, which causes diauxic growth, with each carbon source limiting cell growth. Sufficient supplies of oxygen, ammonium nitrate (NH4NO3) as a nitrogen source and all other macro and micronutrients are available. The bacteria prefer to grow on glucose (C6H12O6, supplied at 80 g/L), so it is consumed first, followed by glycerol (C3H803, supplied at 68 g/L). Since the E. coli were already growing on glucose, the lag time associated with glucose consumption is only 10 minutes. The lag time associated with glycerol consumption is 35 minutes. Further information: The chemical formula for E. coli in this bioreactor is CH1.666N0.2000.57. The specific growth rate on glucose is 0.41 h-1, and on glycerol is 0.32 h-1?The yield of cells on glucose is 0.48 g cells/g glucose, and yield on glycerol is 0.39 g cells/g glycerol. You may assume that…
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