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Substances A and B have retention times of 16.4 and 17.63 min, respectively, on a 30 cm column. An unretained species passes through the column in 1.3 min. The peak width at base for A and B are 1.11 and 1.21 min, respectively. Calculate:
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- (b) For column 1(i) What are the unadjusted retention times for components A and B?Substances A and B have retention times of 12.75 and 14.28 min, respectively, on a 30.0-cm column. An unretained species passes through the column in 1.10 min. The peak widths (at base) for A and B are 1.11 and 1.27 min, respectively. What is the resolution?What is the HETP of a column that is 200mm with a retention time of 5mins. The (effective) theoretical plate number N is 6000. What is the separation factor, a?
- Substances A and B have retention times of 16.40 and 17.63 minutes, in a 30.0 cm column. A species that is not retained passes through the column in 1.30 minutes. The peak widths (at the base) for A and B are 1.11 and 1.21 minutes.Calculate:a) Resolution of the columnb) Average number of plates in the column.c) Height of the plate.d) Time required to elute substance B in a column that gives a resolution value, Rs = of 1.5.Substances A and B have retention times of 16.40 and 17.63 min, respectively,on a 30.0-cm column. An unretained species passes through the column in 1.30 min. The peakwidths (at base) for A and B are 1.11 and 1.21 min, respectively. Calculate(a) the column resolution,(b) the average number of plates in the column,(c) the plate height,(d) the length of column required to achieve a resolution of 1.5.Consider the retention time of a pesticide is.13 8.5 min and the peak width at the base = 1.7 min, length of the column 30 cm, then plate height equals
- The three standards have different retention times on the Mini-GC. What physical characteristics of the molecules do you think contribute to this?From the data calculate: a) retention factor of each solute b) selectivity factor c) how many plates are needed to provide a resolution of 1.5? d)how long a column is needed if the plate height of the packing is 1.5x10^-3cm? e)if the flow rate is 6.75 cm/min how long will it take to elite the two species?A molecular exclusion column has a diameter of 7.8 mm and a length of 30 cm. The solid portion of the particles occupies 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polymers of various molecular masses is eluted between 23 and 27 mL. What does this imply about the retention mechanism for these solutes on the column?
- The separation of APAP-L,(12 = 0.28 min) from APAP-D (W12 = 0.35 min) on a chiral stationary phase was found to have retention times of 4.78 min and 5.26 min, respectively. With a unretained solute being, found to have a retention time of 1.1 min on the same column. Calculate the column resolution and selectivity factorPART A: Substance A was found to have a retention times of 3.15 minutes on a 70.00-cm column. An unretained species passed through the column in 0.50 minutes. The peak width (at base) for A was 0.60 minutes. Using this information, determine the following: 1. What is the retention factor for analyte A? 2. What is the plate count for this column? 3. What is the height equivalent of a theoretical plate? PART B: Match each statement with one of these terms. A Partition coefficient B Mobile phase C Elution D Longitudinal diffusion E Stationary phase F Eddy diffusion G Retention factor H Plate number I Dead time J Retention time K Selectivity factor L Column resolutionTwo substances A and B have retention times of 16.40 and 17.63 min respectively on 30.0 cm column. An unsuccessful species passed over the column in 1.30 min. The widths (at the base) of the peaks ofA and B are respectively 1.11 min and 1.21 min: Calculate: a) The Resolution Rs of the column. b) The average number (Nav of theoretical plates in the column c) The equivalent height (H) d) length of column required to achieve a resolution of 1.5, e) time required to elute substance B on the longer column.
