Compare and contrast Radial Immunodiffusion with it’s close relative, the Ouchterlony plate technique. How are they similar and how are they different?
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Compare and contrast Radial Immunodiffusion with it’s close relative, the Ouchterlony plate technique. How are they similar and how are they different?
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- Why do the ring sizes change until equivalence is reached in radial immunodiffusion?Why monoclonal antibodies method is better than polyclonal antibodies method in detecting specific pathogen? Explain the reasons for both selection or rejection methods..What are the advantages/disadvantages of using a direct immunoblotting method? Compare and contrast to an indirect immunoblotting method.
- a. Seropositivity means having a blood serum that tests positive. Explain why it may or may not develop at the same rate in all patients exposed to the same microbe. b. Would a high rate of false-positives decrease the sensitivity or specificity of a serological test?What component must have been present in the positive control solution (antigen, anti-HIV IgG antibodies, or secondary antibody)? Explain how you know this is true.In radial immunodiffusion what are the circular precipitating rings composed of, and what do they represent?
- Why monoclonal antibodies method is better than Polyclonal Antibodies method in detecting specific pathogen. Explain reasons.Describe the following antigen antibody reactions in serology: Mechanism Function in serology Example of method a) Precipitation b) Agglutination c) Neutralizing d) Complement fixationwrite down the following techniques of immunology and thier applications; 1.precipitation 2.agglutination 3. simple immunodiffusion 4. double immunodiffusion 5.immunoelectrophoresis 6. two dimensial and counter immnuoelectrophoresis 7.immunofluorescence 8.complement fixation
- Why are agglutination tests so widely used in clinicaldiagnostics? How are fluorescent antibodies used todiagnose diseases? What advantages do immunofluorescenttechniques have over traditional culturing?Is rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.Explain what immunoaffinity extraction consists of when applied to obtainingsteroids