Pleas solve ASAP Problem 4A. Consider the enolase reaction 2PG → PEP. Draw the associated structures.B. The standard free energy (@physiological pH, temp, salt) for the 2PG>PEP reaction is +3kJ/mol. Does this mean that net physiological flux is in the direction from PEP to 2PG (Y/N)?Explain your answer (max 1 sentence).C. Briefly explain why the reaction PEP + ADP → Pyr + ATP is thermodynamically favorable (max 2sentences).D. Imagine that you start with 10 mM 2PG (A) and incubate it with enolase (E) and in aqueousbuffer to make PEP (B). Assume the following reaction schemek3E*k4k1A + EВ + Еk2What are suitable units for each of k1, k2, k3, and k4?E. Write down equations for dA/dt, dB/dt, and dE*/dt in terms of E and E* where A, B, C, E, E* areall concentrations and E is free enzyme.F. Write down an equation relating E and E* to Eo (initial concentration of E added to the tube).G. Set E* at steady-state. Assume A and B are much more abundant than enzyme (A,B >> E,). Solvefor E and E* in terms of A, B, and Eo and the k values. (Writing-saving hint: you may wish todefine X = k1A + k2 + k3 + k4B.)H. Solve for dA/dt in terms of A, B, and Eo and the k values.1. Solve for Keq in terms of k1, k2, k3, and k4.J. If (in some unit scheme that you defined above) the numerical values of k1, k2, and k3 are 10, 1,and 10, what is the approximate value of k4?K. What are the approximate concentrations of 2PG and PEP at equilibrium?L. You now start with 10 mM PEP and incubate it with enolase and in aqueous buffer to make 2PG.What are the approximate concentrations of 2PG and PEP at equilibrium?

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Consider the enolase reaction 2PG PEP. Draw the associated structures. The standard free energy (@physiological pH, temp, salt) for the 2PG>PEP reaction is +3 kJ/mol. Does this mean that net physiological flux is in the direction from PEP to 2PG (Y/N)? Explain your answer (max 1 sentence). Briefly explain why the reaction PEP + ADP → Pyr + ATP is thermodynamically favorable (max sentences).