Contigs are often made using BAC or cosmid vectors. What are theadvantages and disadvantages of these two types of vectors? Whichtype of contig would you make first, a BAC or cosmid contig? Explain.

Q: name  the six different types of vectors?

A: Vector is DNA molecule that is used as a vehicle to artificially carry the gene of interest using…

Q: What would be the most likely effect on fruit-fly development of a deletion in the nanos gene?

A: Drosophila melanogaster is a fly species in the family Drosophilidae. This fly is commonly called as…

Q: If the heavy chain of an antibody is approximately 450 amino acids long, how much DNA would be…

A: In this question we are asked that how much DN A may be possibly required to encode 10^0 split heavy…

Q: When researchers cloned the ey, Pax-6, and AN genes, What did they found?

A: Introduction Pax-6 stands for Paired Box Protein-6. Pax is the group of gene that lead a main role…

Q: What is a filtered word test?

A: A Screening test to asses central auditory processing disorder in children of 5-11 years, consists…

Q: What three essential criteria must be met in order to execute asuccessful mapping cross?

A: Introduction: A genetic cross is the mating of two individuals with desired characters. The…

Q: A newly developed qPCR has an efficiency of 75%, and each cycle is pretty consistent. In this qPCR,…

A: The qPCR is generally useful to determine the actual value of PCR product present at provided cycle.…

Q: What is the molecular characteristic and phenotype of the silent mutation in multidrug resisitent…

A: Silent mutation are the mutations in DNA which do not have an phenotypic effect on the organism.…

Q: Briefly describe two approaches that are used to edit the germline in embryos using CRISPR-Cas9.…

A: The mechanism of CRISPR/Cas-9 genome editing can be generally divided into three steps: recognition,…

Q: What is The Luria-Delbrück fluctuation experiment?

A: Mutations are defined as any change in the nucleotide sequence that also alters the phenotype of the…

Q: What is the difference between a Lambda  insertion vector such as Lambda gt11 and a Lambda…

A: Step 1 Cloning vectors are carriers or vehicles of desired DNA (deoxyribonucleic acid) fragments…

Q: Mention any three vector-less methods that are used to introduce recombinant DNA into a competent…

A: Micropropagation ,electroporation and gene gun methods are three vectorless methods that are used to…

Q: How can the construct with Cas9 and gRNA sequence be removed from the plant in case it was inserted…

A: The generation of new plants with improvised constructs of features that are desirable is based on…

Q: What would be the effect on X inactivation of adding siRNAs that eliminated the products of each of…

A: X-inactivation is the process in which one of the X-chromosome in females is inactivated…

Q: A newly developed qPCR has an efficiency of 75%, and each cycle is pretty consistent. In this qPCR,…

A: Probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a…

Q: Using blue-white selection, explain how to discriminate against bacteria with self-ligated vectors.…

A: Blue white selection is an efficient technique for the identification of Recombinant bacteria in a…

Q: What does the following vector produce? Be specific.

A: Vector is defined as a DNA molecule that is used as a vehicle to carry foreign genetic material into…

Q: Would the application of paclitaxel to a cell make us incapable of performing karyotyping on that…

A: Karyotyoing is the process by which DNA is analyzed . The chromosome structure is usually studied .

Q: A donor strain of bacteria with alleles a+ b+ c+ is infected with phages to map the donor chromosome…

A: Bacteria are unicellular and prokaryotic organisms that live in wide variety of environments. It…

Q: How does homologous recombination with transfected disruption constructs can inactivate specific…

A: The plasmid does not contain the yeast origin of replication. The gene target constructed to…

Q: What is cotransduction? What determines the likelihood that two genes will be cotransduced?

A: We have to have the knowledge of genetics to answer this question.

Q: Mention the important properties which a good vector must possess?

A: The vectors that are commonly used in the field of genetic engineering possess certain features that…

Q: What is the difference between a l insertion vector such as lgt11 and a l replacement vector?

A: Vector in molecular biology is a DNA molecule which carries foreign genetic material into foreign…

Q: What is the difference between generalized and specialized transduction?

A: Transduction is a method of gene transfer in bacteria from donor to recipient using bacteriophage.…

Q: Why is it that somatic gene therapy is allowed in many countries and yet germ-line gene therapy is…

A: Gene therapy is a technique in which genes are used for the prevention and treatment of disease.…

Q: "Genome-Wide Association Studies Identify Genome Variations That Contribute to Disease" Explain this…

A: The genome-wide association comprises scanning all the marker genomes or complete sets of DNA to…

Q: What transcritpion factor is produced in the Nieuwkoop center in reponse to nuclear beta-catenin?(…

A: Transcription factors are usually the protein that have the capacity to increase or decrease the…

Q: Explain the Choice of vectors?

A: Cloning Vectors are small pieces of DNA taken from a plasmid, virus, bacteria, and cell of any…

Q: Describe the effect of the deletion on co-transformation of pure and pepN genes that are isolated…

A: Transformation is a process of transforming the genes of one strain and then transferring it to…

Q: What sorts of things can we do to help prevent ourselves from catching COVID-19? Explain why these…

A: Coronavirus belongs to the family of Coronaviridae that causes upper respiratory infection. In 2019…

Q: How has antagonistic pleiotropy been used during the development of vaccine candidates?

A: Pleiotropy : When one gene controls effect of more than one phenotypic character in an organism.…

Q: What kind of vector is this?

A: This is the picture of human flea , Pulex irritans.  It is very poor vector.  It cause trench…

Q: What is the major difference between generalized transductionand transformation?

A: TRANSDUCTION:- Is the process  by which DNA is transferred from one bacterium to another by a virus…

Q: What is an experiment  that could be perform to differentiate between a gibberellin-insensitive…

A: Gibberllin is a plant hormone that is responsible for the elongation of stems and the development of…

Q: During the [X) phase of CRISPR-Cas9 defense, sequences from a new bacteriophage that infects the…

A: Clustered regularly interspaced short palindromic repeats abbreviated as CRISPR refers to a family…

Q: Why is generalized transduction able to transfer anygene, but specialized transduction is restricted…

A: Introduction: transduction is a process of gene transfer in bacteria from donor to recipient…

Q: If you were to engineer some non-adhesive cells to express E-cadherin at either high levels or low…

A: Introduction: E- Cadherin is a type of cell adhesion molecule (CAM) that are important in the…

Q: If a soluble protein cannot be obtained from a bacterial expression vector, how else can you produce…

A: Soluble proteins are those with a solubility of more than 70% and insoluble with a solubility of…

Q: In a particular species, the gene for the kappa light chain has 200 V segments and 4 J segments. In…

A: Immune system protects the body against infection. It is a complex network of cells and proteins.…

Q: How do you design a reporter assay for Serpina1?

A: The gene has two parts. The first is the coding sequence which gets transcribed to RNA and…

Q: Explain why mRNA is preferred for making COVID-19 vaccines?

A: In general, a weakened or attenuated form of the pathogen is used as a vaccine to activate the…

Q: What is the Use of a transgene expressing hypermorphic Ras in a modifier screen?

A: A modifier screen is a kind of screen with pre‐existing aggregates that are used to distinguish…

Q: What determines the likelihood that two genes will be cotransduced?

A: To determine the likelihood that two genes will be cotransduced

Q: can BRCA1 null mutant transcribe any genes

A: The genes BRCA1 and BRCA2 (BReast CAncer genes 1 and 2, respectively) generate proteins that help in…

Q: What is Ponceau S and why do we use it during western blotting?

A: Western Blotting : It is an analytical technique of molecular biology which is used to separate the…

Q: What must a vector contain?

A: Vector is a nucleic acid molecule containing Viral DNA or RNA and plasmid yeast or bacterial…

Q: In the gene therapy trials for SCIDS, what DNA was added to the patients’ cells? Could this same…

A: "Severe combined immunodeficiency" (SCID) is an X-linked disorder of the immune system that occurs…

Q: What is the visibility of proteomic phenotype in COVID-19 patients?

A: The protein is defined because the set of proteins that an organism will manufacture. By…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.