Q: The map of the lac operon is shown below. Consider the following examples that include both haploids…
A: Lac operon is the segment of DNA which includes regulatory gene, structural gene, promoter gene and…
Q: What is an exconjugant? How do you think that exconjugants were obtained? (It might include genes…
A: Answer: Introduction: Conjugation is the process of transfer of DNA from one bacterium to other by…
Q: What would be the most likely effect on fruit-fly development of a deletion in the nanos gene?
A: Drosophila melanogaster is a fly species in the family Drosophilidae. This fly is commonly called as…
Q: What is micropropagation? Describe the different in-vitro stages in the micropropagation of any…
A: Plant tissue culture is a technique in plant biotechnology. This technique involves the culturing of…
Q: For what the genetically modified brinjal has been developed in india?
A: The genetically modified brinjal has been developed in India for giving resistance against…
Q: What experiment could you perform to differentiate between a gibberellin-insensitive mutant and a…
A: Gibberellin is a plant hormone. Regulate various developmental and physiological processes in…
Q: How reliable does the rbcL gene work as a barcode for identifying plant species?
A: * DNA barcoding is method to identify a species by using a short section of DNA. They will compare…
Q: Define the terms prototroph and auxotroph.
A: A medium containing inorganic compounds but no organic compounds other than a carbon source is…
Q: What are the important steps in the isolation of pectin from citrus peel?Why each steps are…
A:
Q: Using blue-white selection, explain how to discriminate against bacteria with self-ligated vectors.…
A: Blue white selection is an efficient technique for the identification of Recombinant bacteria in a…
Q: Insert a specific stress-tolerant gene into a tissue cultured plant batch. What can be your possible…
A: The method includes selecting a test plant in which the stress-tolerant gene is to be added. First…
Q: Explain about auxotroph. Define its working ?
A: Auxotroph are microorganisms that are unable to synthesize an essential nutrient because of gene…
Q: bacteria
A: Auxotrophic strains lack the ability to synthesize one essential compound for example amino acid.…
Q: What is the aim of extracting DNA of a strawberry?
A: Deoxyribonucleic acid (DNA) is a genetic material containing thousands of genes which code for…
Q: Describe how you would genetically design a superbug resistant to β-lactams, methicillin,…
A: Superbugs are varieties of bacteria, viruses, parasites, and fungi that can survive most antibiotics…
Q: What is an interrupted mating experiment? What type of experimentalinformation can be obtained from…
A: Interrupted mating is a method used to MAP bacterial qualities by deciding the arrangement in which…
Q: What are ‘Selectable marker’? What is their use in genetic engineering?
A: Genetic engineering is a technique to manipulate the genetic content of an organism resulting in…
Q: How does homologous recombination with transfected disruption constructs can inactivate specific…
A: The plasmid does not contain the yeast origin of replication. The gene target constructed to…
Q: Compare and contrast the use of histochemical reporter genes and fluorescent reporter genes — what…
A: Reporter genes can be analyzed by different methods, such as beta glucuronidase (uidA) gene…
Q: What is o A vector derived from the tumor-inducing (Ti) plasmid of the bacterium Agrobacterium…
A: In recombinant technology, vectors are the DNA molecules that are used as a vehicle which carry…
Q: What is using somatic nuclei of transgenic adults to generate other animals with identical genomes?
A: Genomics refers to structure, function, evolution, mapping, and editing of genomes. Somatic cells…
Q: Why is replica plating used to isolate an auxotrophic mutant from a prototrophic parent?
A: Auxotrophs are the organisms that are unable to synthesize a particular organic compound necessary…
Q: Explain how the analysis of arginine auxotrophs implied that a single gene corresponds to a single…
A: The genes are the components of the genome that direct specific functions in the cell. Their…
Q: How does an auxotroph differ from a prototroph in bacteria?
A: Microorganisms display different types of nutrition. Although some bacteria live in symbiotic…
Q: Why is the use of auxotrophy not a good method for controllingthe growth of a genetically modified…
A: Genetically modified organisms are those living organisms which have been genetically modified by…
Q: Describe the effect of the deletion on co-transformation of pure and pepN genes that are isolated…
A: Transformation is a process of transforming the genes of one strain and then transferring it to…
Q: Describe the Ti plasmid binary vector system used in plant transformation and provide details of how…
A: Plasmids are small circular double-stranded DNA molecules present in bacterial cells and other…
Q: Describe direct selection and indirect selection of identifying mutants. What are auxotroph and…
A: In the direct selection method, a population of mutagenized bacterial cells is inoculated into a…
Q: What is Auxotroph and Prototroph?
A: Microorganisms are microscopic organisms, which are unicellular, multicellular, or cell clusters.…
Q: What is special about the EP line? How do we identify and monitor the EP line in transgenic flies?
A: Genes are organised to make gene expression regulation simpler.
Q: Time mapping is performed in a cross involving the genes his,leu, mal, and xyl. The recipient cells…
A: Conjugation occurs between two dimorphic bacteria where one is a donor and the other is a recipient.…
Q: Is the genus alcaligenes gelatin negative or postive?
A: Prokaryotes are single-celled microscopic organisms belongs to the domain bacteria. A prokaryotic…
Q: What are the different vectors available to conduct plant genetic transformation?
A: Vectors for plant genetic transformation.
Q: What kind of vector is this?
A: This is the picture of human flea , Pulex irritans. It is very poor vector. It cause trench…
Q: Researchers can introduce loss-of-function mutations into genesusing the CRISPR/Cas9 technology .…
A: The genetic mutations that alter the morphological characteristics of plants such as the flowers,…
Q: Describe how you would genetically design a superbugresistant to b-lactams, methicillin,…
A: Superbugs are strains of viruses, bacteria, fungi, and parasites that are resistant to most the…
Q: What is the difference between a transgenic plant and a plant produced through selective breeding?
A: Selective breeding entails pairing up parents with similar characteristics in order to create kids…
Q: If a winter-annual strain of Arabidopsis is grown in a greenhouseand not exposed to cold…
A: Arabidopsis, also called rockcress, are small flowering plants. They belong to the family…
Q: (a) What do reverse transcriptases do? Give two examples of these and what they do in the cell.…
A: Recombinant DNA technology involves the insertion of a transgene into a microorganism. Polymerase…
Q: How is an auxotroph different from a prototroph?
A: Introduction: Auxotrophs are mutant organisms generally fungus or bacterium that need a special…
Q: To produce transgenic plants, plant tissue is exposed to Agrobacteriumtumefaciens and then grown in…
A: Agrobacterium tumefaciens is usually used in the plant genetic transformations. The desired gene is…
Q: Why are the recombinants produced from an Hfr * F- cross rarely, if ever, F+?
A: An Hfr cell is a bacterium with a conjugative plasmid (such as the F-factor) incorporated into its…
Q: In a gene mapping experiment, DNA from a prototrophic E. coli strain is transformed into an E. coli…
A: The autotrophic microorganisms are mutant for any one or more growth factors. They are incapable of…
Q: the role of X-gal? What color will cells with the plasmid be in the absence of X-gal? Why?
A: X- gal refers to the organic compound which comprises a galactose associated with an indole group.…
Q: How does an auxotroph differ from a prototroph?
A: Microorganisms grow on different nutrient sources. Some are adapted to grow well in both the minimal…
Q: is “Agrobacterium-mediated genetic transformation” in plants described as natural genetic engineer…
A: Agrobacterium is a gram-negative bacteria that uses horizontal gene transfer to cause tumors in…
Q: Describe how replica plating is used to detect and isolate auxotrophic mutants.
A: Auxotrophic Mutant:Auxotrophic mutants are strains that require a growth supplement that the…
Q: Name on methoud that could have been used to deterine where the DMR are in the figures. And How is…
A: *DNA methylation is an epigenetic mechanisms that involves in many pathogenic processes for…
What is an experiment that could be perform to differentiate between a gibberellin-insensitive mutant and a gibberellin auxotroph?
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- Why are mutations in the INK4 locus so dangerous?Your supervisor asked you to insert a specific stress-tolerant gene into a tissue cultured plant batch. What can be your possible approach? You may only give a flow chart to show your process.Insert a specific stress-tolerant gene into a tissue cultured plant batch. What can be your possible approach? You may only give a flow chart to show your process.
- With the technique of interrupted mating four Hfr strains were tested for the sequence in which they transmitted a number of different genes to an F strain. Each Hfr strain was found to transmit its genes is a unique sequence, as shown in the accompanying table. What is the gene sequence in the original strain from which these Hfr strains were derived?What is Auxotroph and Prototroph?The synthesis of flower pigments is known to be dependent on enzymatically controlled biosynthetic pathways. For the crosses shown here, postulate the role of mutant genes and their products in producing the observed phenotypes: (a) P1: white strain A * white strain B F1: all purple F2: 9/16 purple: 7/16 white (b) P1: white * pink F1: all purple F2: 9/16 purple: 3/16 pink: 4/16 white