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Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Could someone explain what this is saying? I would like a better understanding. FIGURE D

CPP32 (Caspase-3) protease activity. Aliquots of cell lysates (50
μL) were incubated with an equal volume (50 μL) of the reaction
buffer and 5 μL of 7-amino-4-trifluoromethyl coumarin (AFCDEVD) for 1 hour at 37˚C (Clontech). The shift in fluorescence
emission of AFC-DEVD, on its proteolysis to free AFC by the
protease, was detected in a fluorometer, using a 400 nm excitation
filter and 505 nm emission filter.

a
180
160
160
140
140
120
120
100
100
80
80
60
60
40
40
20
20
1
3
10
30
100
1
3
10
30
100
Sodium ascorbate (mM)
Sodium ascorbate (mM)
d
160
600
140
500
120
400
100
300
80
60
200
40
100
20
1
3
100
1
10
30
100
Sodium ascorbate (mM)
Sodium ascorbate (mM)
Bax (% of control)
p21 (% of control)
*
Caspase-3 (% of control)
p53 (% of control)

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